RT - European Journal of Gynaecological Oncology ID - 10.12892/ejgo20100155 T1 - Analysis of protein profiles in human epithelial ovarian cancer tissues by proteomic technology A1 - S.N. Chow A1 - R.J. Chen A1 - C.H. Chen A1 - T.C. Chang A1 - L.C. Chen A1 - W.J. Lee1 A1 - J. Shen A1 - L.P. Chow K1 - Ovarian cancer; Proteomics; Two-dimensional gel electrophoresis; Matrix-assisted laser desorption/ionization-time of fight mass spectrometry. YR - 2011 SP - 55 AB -

Background: Screening in ovarian cancer is progressively finding out candidate genes and proteins which may work as screening biomarkers and play a role in tumor progression. We examined the protein expression patterns of ovarian cancer tissues using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of fight mass spectrometry (MALDI-TOF MS). Methods: Tissues from 36 ovarian cancers and 20 normal ovaries were examined by 2-DE. The images of silver stained gels were analyzed by ImageMaster 2D Elite. The peptide mixtures, after ingel digestion, were determined by MALDI-TOF MS for fingerprinting. The deisotope tryptic peptide profiles were matched by using the Mascot search engine based on the entire NCBI and Swiss-Prot protein databases. Western/dot blots were then applied to verify the findings. Results: In ovarian cancer, 12 proteins that showed differential expressions were identified unequivocally. Among these proteins, five proteins (galectin-1, cathepsin B, ubiqui-tin carboxy-terminal hydrolase L1, HLA class II antigen DRB1-11 and heat shock protein 27) were up-regulated and seven proteins (cellular retinolbinding protein, transthyretin, SH3 binding glutamic-rich-like protein, tubulin-specific chaperone A, DJ-1, gamma-actin and tropomyosin 4) were down-regulated. Conclusion: The present study is the first to report the up-regulation of ubiquitin car-boxy-terminal hydrolase L1 and the down-regulation of SH3 binding glutamic-rich-like protein, tubulin-specific chaperone A, and tropomyosin 4 in human ovarian cancer tissues. Further cloning and functional analysis of these salient proteins will provide more information on their pathophysiologic roles in ovarian cancer.