The aim of this study was to evaluate the impacts of fertility-preserving surgery and chemotherapy on the fertility and ovarian functions in malignant ovarian cancer. The clinical data of 52 female patients with malignant ovarian cancer and performed fertility-preserving treatment (FPT) in the present hospital between June 2004 and December 2014 were retrospectively analyzed. The 52 cases included 25 cases of germ cell carcinoma (GCC) (48.07%), 12 cases of epithelial ovarian carcinoma (EOC) (23.07%), and 15 cases of borderline carcinoma (28.84%), among which one case recurred (1.92%) and one died (1.92%). Forty-five cases had normal menstruation after the treatment (86.53%), and 16 cases out of the 27 cases with fertility requirement successfully achieved pregnancy (59.26%). FPT in female patients with malignant ovarian cancer was effective and feasible; although chemotherapy might affect ovarian function, it could also be reversed. Ovarian tumor resection or adnexectomy may be the best way for fertility preserving treatments; however, further investigation is warranted.