RT  - European Journal of Gynaecological Oncology
ID  - 10.12892/ejgo3990.2018
T1  - Effect of siRNA against nerve growth factor (NGF) on growth and invasion of ovarian cancer cell
A1  - Fengjuan Liu
A1  - Hong Tao
A1  - Qi Shen
A1  - Yinglin Zheng
A1  - Ke Zhang
A1  - Jinyi Tong
K1  - NGF; NGF siRNA; TrkA; p57; VEGF; Ovarian cancer.
YR  - 2018
SP  - 470
AB  - <p>normal ovaries and in epithelial ovarian carcinomas. To examine the effects on ovarian cancer cell line (A2780) proliferation, invasion, and apoptosis after NGF down-regulation induced by lentivirus induced RNAi. Materials and Methods: The expression and localization of NGF, TrkA, p75, and VEGF in normal ovarian samples and in ovarian cancer samples were analyzed by RT-PCR and immunohistochemistry. NGF knockdown in A2780 was achieved by shRNA and cell proliferation, invasion, and apoptosis was examined by MTT, transwell assay, and flow cytometry, respectively. Results: Significantly higher levels of NGF, TrkA, and VEGF were observed in ovarian cancers versus normal ovary, while expression level of p75 maintained stable. In A2780 cells, NGF down-regulation significantly reduced the expression levels of TrkA and VEGF, but did not change that of p75. A significant decrease in proliferation and invasion, as well as an increase in apoptosis was also observed in NGF down-regulation cells. Conclusions: The expression of TrkA and VEGF is importantly induced by NGF. Knockdown of NGF promoted apoptosis, thus inhibited cellular proliferation and invasion in A2780 cells. Therefore NGF block may be a potential therapeutic strategy to treat ovarian cancer.</p>