Vitexin is widely known as a bioactive flavonoid compound, and exerts analgesic and anti-oxidative properties. Antitumor effect of vitexin was identified in various tumors. Role and related mechanism of vitexin in cervical cancer were investigated. Cervical cancer cells were treated with vitexin, and treatment with vitexin reduced cell viability, and decreased number of colonies in cervical cancer. Moreover, cell migration and invasion were repressed by vitexin, and vitexin inhibited angiogenesis of cervical cancer. Vitexin reduced protein expression of Vascular Endothelial Growth Factor A (VEGFA) and Vascular endothelial growth factor receptor 2 (VEGFR2) of cervical cancer in a dosage dependent way. In conclusion, vitexin reduced cell proliferation, migration, invasion and angiogenesis of cervical cancer through inhibition of VEGFA/VEGFR2 signaling.