It is unknown what part Polyphyllin VII plays in HumanPapillomavirus (HPV)-positive cervical cancer, despite its recognized inhibitory effects on several types of cancer. The main focus of this work is to examine how Polyphyllin VII affects the proliferation and death of HPV-positive cervical cancer cells. To begin with, Hela and Caski cells were treated with varying doses of Polyphyllin VII. Subsequently, cell counting kit-8 (CCK-8) was used to evaluate the viability. Cell proliferation, cloning, cycle and apoptosis were analysed by flow cytometry and Western blot analysis was used to look at protein expression related to the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/Akt) pathway. The results showed that medium and high concentrations of Polyphyllin VII treatment of HPV-positive cells caused a notable decline in cell viability and crystal violet number, and prevented cells from transitioning from G1 phase to S phase. The rate of apoptosis also increased significantly, and there was a noticeable decline in the expression of p-PI3K/PI3K and p-Akt/Akt. Thus, it is concluded that medium and high concentrations of Polyphyllin VII inhibit the proliferation of HPV-positive cells and cause both apoptosis and cell cycle halt. Polyphyllin VII impedes the proliferation of HPV-positive cervical cancer cells through the PI3K/Akt pathway.