Micro RNAs (miRNAs) contribute to the progression and expansion of many cancers, including cervical cancer. Reports reveal the down-regulation of MicroRNA-375 (miR-375) within cervical cancer tissues. Nevertheless, the mechanism for regulation of cervical cancer progression by miR-375 is still unknown. The current work deals with the molecular mechanisms orienting the regulatory impacts of miR-375 on invasion and migration in human cervical carcinoma. miRNA expression within cervical cancer tissues was assessed through Stem-loop RT-PCR. The invasion and migration of miR-375 mimic-transfected SiHa cells were observed by the Transwell test and wound healing test, respectively. Bioinformatics analysis was performed for predicting the target gene of miR-375, which was validated by the dual-luciferase test. Real-time RT-PCR and Western blot analysis were utilized for detecting protein expression and mRNA transcription. In cervical cancer, miR-375 expression was 4.2 ± 0.15-fold lower compared to the adjacent noncancerous tissues. The number of invasive and migrated SiHa cells (32 ± 3.6; 21.86 ± 6.4) transfected with miR-375 mimics were considerably lower in comparison with the NC group (101 ± 7.5; 41 ± 5.6) after 24 hours. Real-time RT-PCR and Western blot analysis confirmed JAK2 as a downstream target gene of miR-375. The protein expression and mRNA level of JAK2 in miR-375 mimic-transfected SiHa cells were decreased. The present study indicated that the invasion and migration of SiHa cells are regulated by miR-375 through down regulating JAK2 expression. This study provides a theoretical and experimental basis for reversing the metastasis of cervical cancer and developing new candidate drugs.