RT  - European Journal of Gynaecological Oncology
ID  - 10.31083/j.ejgo4302033
T1  - Interaction of Pten gene and AKT/mTOR pathway in endometrial adenocarcinoma proliferation
A1  - Dan Tang
A1  - Ming-Rong Xi
A1  - Xi Zeng
K1  - Pten/AKT/mTOR pathway; endometrial cancer; tumor proliferation
YR  - 2022
SP  - 265
AB  - <p><strong>Objective</strong>: The Pten/AKT/mTOR pathway is one of the most critical pathways in tumor proliferation. The present research aimed to analyze the interaction between <i>Pten</i> gene and AKT/mTOR pathway in endometrial cancer cell lines. <strong>Methods</strong>: TCGA analysis was used to study the relationship between <i>Pten</i> expression and survival of endometrial cancer patients. Human endometrial cancer cell lines with low <i>Pten</i> expression (Ishikawa) and with high <i>Pten</i> expression (HEC-1-A) were selected. Plasmid transfection was used to regulate the <i>Pten</i> expression in the cell lines. QRT-PCR and Western Blot were adopted to detect Pten/AKT/mTOR expressions in tumor cells. Western blot of Ki-67 and CCK-8 were adopted to detect the activity of cells proliferation. A <i>p </i><inline-formula>&lt;</inline-formula> 0.05 was considered to be statistically significant. <strong>Results</strong>: The TCGA analysis showed the <i>Pten</i> expression was associated with survival of endometrial cancer patients significantly. Plasmid transfections elevated <i>Pten</i> expression in Ishikawa and decreased <i>Pten</i> expression in HEC-1-A cells. After the plasmid transfection, with overexpression of <i>Pten</i> in Ishikawa cell line, the Western Blot and QRT-PCR revealed the AKT/mTOR pathway is restrained, leading to decreased cell proliferation; with <i>Pten</i> decreased in HEC-1-A cells, the AKT/mTOR pathway is activated, leading to increased cell proliferation. <strong>Conclusions</strong>: A decreased expression of <i>Pten</i> gene in Ishikawa and HEC-1-A cell lines could activate AKT/mTOR pathway and promote tumor cells proliferation.</p><p><br></p>