Objective: The molecular mechanism in the occurrence and progression of cervical intraepithelial neoplasia (CIN) is of great theoretical significance to improve the early diagnosis, treatment and prognostic judgement of CIN in clinical practice. The current study aimed to detect the expression of remodel and space factor-1 (Rsf-1) and P16 in CIN, and to analyze the expression of Rsf-1 and P16 in different stages of CIN and to investigate the possible correlation. Methods: A total of 110 samples of CIN were collected from the Department of Pathology, the First Affiliated Hospital of Bengbu Medical College in 2018. Rsf-1 and P16 was detected by immunohistochemical staining in pathological tissue sections. The correlation between Rsf-1 expression and P16 expression in CIN was further analyzed. Results: The immunohistochemical results of 110 cases of CIN showed that the positive rate of P16 and Rsf-1 gradually increases with the grade of CIN increases. The expression of P16 was significantly different between different CIN grade (p = 0.011). The expression of Rsf-1 was also significantly different in different CIN grade (p = 0.015). Conclusions: Rsf-1 and P16 was highly expressed or over-expressed in high-grade CIN, and the positive rate of their expression increased with the increase in CIN grade.