Article Data

  • Views 381
  • Dowloads 137

Original Research

Open Access

The role of p53, Bcl-2 and Ki-67 in premalignant cervical lesions and cervical cancer

  • I. Turkcuoglu1,*,
  • S. Tezcan1
  • G. Kaygusuz2
  • C. Somer Atabekoglu
  • F. Ortac1
  • M. Giingor1
  • D. Kankaya2
  • A. Sertcelik2

1Department of Obstetrics and Gynecology, Turkey

2Department of Pathology, Ankara University School of Medicine, Ankara, Turkey

DOI: 10.12892/ejgo200704290 Vol.28,Issue 4,July 2007 pp.290-293

Published: 10 July 2007

*Corresponding Author(s): I. Turkcuoglu E-mail:

Abstract

Purpose: The aim of this study was to determine the role of p53, Bcl-2 and Ki-67 expression in the carcinogenesis of cervical carcinoma and aggressiveness of cervical intraepithelial neoplasia (CIN).

Methods: The pathology specimens of 63 patients with a diagnosis of normal squamous epithelium (22 cases), CIN I (14), CIN II (5), CIN III (8) and squamous cell carcinoma (14) were evaluated immunohistochemically for the expression of p53, Bcl-2 and Ki-67 in paraffin sections.

Results: The expression of p53 and Ki-67 increased proportionally to the grade of CIN and cervical cancer, but only the increase of p53 expression was statistically significant (p = 0.002). There was no significant correlation between Bcl-2 expression and premalignant and malignant cervical lesions.

Conclusion: p53 expression may have a role in the carcinogenesis of squamous cell cervical carcinoma whereas Bcl-2 expression has no role. Ki-67 expression can not be used in determining the aggressiveness of CIN lesions.

Keywords

Cervical intraepithelial neoplasia; Cervical cancer; p53; Bcl-2; Ki-67

Cite and Share

I. Turkcuoglu,S. Tezcan,G. Kaygusuz,C. Somer Atabekoglu,F. Ortac,M. Giingor,D. Kankaya,A. Sertcelik. The role of p53, Bcl-2 and Ki-67 in premalignant cervical lesions and cervical cancer. European Journal of Gynaecological Oncology. 2007. 28(4);290-293.

References

[1] Van Leeuwen A.M., Pieters W.J., Hollema H., Burger M.P.: "Atypical mitotic figures and the mitotic index in cervical intraepithelial neoplasia". Virchows Arch., 1995, 427,139.

[2] Schluter C., Duchrow M., Wohlenberg C., Becker M.H., Key G., Flad H.D. et al.: "The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins". J. Cell Biol., 1993, 123, 513.

[3] Kruse A.J., Baak J.P., de Bruin P.C., Jiwa M., Snijders W.P., Boodt P.J. et al.: "Ki-67 immunoquantitation in cervical intraepithelial neoplasia (CIN): a sensitive marker for grading". J Pathol., 2001, 193, 48.

[4] Bar J.K., Harlozinska A., Sedlaczek P., Kasiak J., Markowska J. "Relations between the expression of p53, c-erbB-2, Kl-67 and HPV infection in cervical carcinomas and cervical dysplasias". Anticancer Res., 2001, 21, 1001.

[5] Cheah P.L., Looi L.M.: "p53 immunohistochemical expression messages in cervical carcinogenesis". Pathology, 2002, 34, 326.

[6] Giarnieri E., Mancini R., Pisani T., Alderisio M., Vecchione A "Msh2, Mlhl , Fhit, p53, Bcl-2, and Bax expression in invasive and in situ squamous cell carcinoma of the uterine cervix". Clin. Cancer Res., 2000, 6, 3600.

[7] Dimtrakakis C., Kymionis G., Diakomanolis E., Papaspyrou I., Rodolakis A., Arzimanoglou I. et al.: "The possible role of p53 and bcl-2 expression in cervical carcinomas and their premalignant lesions". Gynecol. Oncol., 2000, 77, 129.

[8] Dellas A., Schultheiss E., Holzgreve W., Oberholzer M., Torhorst J., Gudat F.: "Investigation of the Bcl-2 and C-myc expression in relationship to the Ki-67 labelling index in cervical intraepithelial neoplasia". Int. J. Gynecol. Pathol., 1997, 16, 212.

[9] Hollstein M., Sidransky D., Vogelstein B., Harris C.C.: "p53 mutations in human cancers". Science, 1991, 253, 49.

[10] Vogelstein B.: "Cancer. A deadly inheritance". Nature, 1990, 348, 681.

[11] Hunt C.R., Hale R.J., Buckley C.H., Hunt J.: "p53 expression in carcinoma of the cervix". J. Clin. Pathol., 1996, 49,971.

[12] Ngan H.Y., Liu S.S., Yu H., Liu K.L., Cheung A.N.: "Proto-oncogenes and p53 protein expression in normal cervical stratified squamous epithelium and cervical intra epithelial neoplasia". Eur. J. Cancer, 1999, 35, 1546.

[13] Tjalma W.A., Weyler J.J.. Bogers J.J.. Polleflet C., Baay M., Goovaerts G.C. et al.: "The importance of biological factors (Bcl-2, bax, p53, PCNA, MI, HPV and angiogenesis) in invasive cervical cancer". Eur. J. Obstet. Gynecol. Reprod. Biol., 2001, 97, 223.

[14] Chen H.Y., Hsu C.T., Lin W.C., Tsai H.D., Chang W.C.: "Prognos tic value of p53 expression in Stage IB 1 cervical carcinoma" Gynecol. Obstet. Invest., 2000, 49, 266.

[15] Finlay C.A., Hinds P.W., Tan T.H., Eliyahu D., Oren M., Levine A.J.: "Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life". Mal. Cell. Biol., 1988, 8, 531.

[16] Kastan M.B., Onyekwere O., Sidransky D., Vogelstein B., Craig R.W.: "Participation of p53 protein in the cellular response to DNA damage". Cancer Res., 1991, 51, 6304.

[17] Prives C., Hall P.A.: "The p53 pathway". J. Pathol., 1999, 187, 112.

[18] Reed J.C.: "Bcl-2 and the regulation of programmed cell death" J. Cell. Biol., 1994, 124, 1.

[19] Cooper K., Haffajee Z., Taylor L.: "bcl-2 immunoreactivity, human papillomavirus DNA, and cervical intraepithelial neoplasia". Mod. Pathol., 1999, 12, 612.

[20] Kokawa K., Shikone T., Otani T., Nakano R.: "Apoptosis and the expression of Bax and Bcl-2 in squamous cell carcinoma and adenocarcinoma of the uterine cervix". Cancer, 1999, 85, 1799.

[21] Cheung T.H., Chung T.K., Lo KW., Yu M.Y., Krajewski S., Reed J.C. et al.: "Apotosis-related proteins in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix". Gynecol. Oncol., 2002, 86, 14.

[22] Chung T.K.H., Cheung T.H., Lo W.K., Yim S.F., Yu M.Y., Krajewski S. et al.: "Expression of apoptotic regulators and their significance in cervical cancer" (letters). Cancer, 2002, 180, 63.

[23] ter Harmsel B., Kuijpers J., Smedts F., Jeunink M., Trimbos B., Ramaekers F.: "Progressing imbalance between proliferation and apoptosis with increasing severity of cervical intraepithelial neoplasia". Int. J. Gynecol. Pathol., 1997, 16, 205.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Submission Turnaround Time

Conferences

Top