Detection of Chlamydia Trachomatis infection in women with CIN and invasive carcinoma. Controversial results of different methods

Chlamydia (Ch.) trachomatis infection as a sexually transmitted disease is highly important, but reliable methods of diagnosing it remain to be worked out. We used three methods of detection: an immunoenzymatic technique for detection of Ch. trachomatis antigen in endocervical material, in situ PCR, and enzyme-immuno assay for detection of IgG class anti-Ch. trachomatis antibodies in serum. We have compared the IS-PCR technique and method of detection of the endocervical antigen. We have not confirmed compatibility of the results obtained in these two methods. Parallel positive results obtained in patient serum and detection of chlamydial DNA by IS-PCR have been accepted to be indicative of persistent infection of Ch. Trachomatis.

Ch. Trachomatis; IS-PCR

[1] Smith T. F.: "Chlamydie". In: "Clinical and Pathogenic Microbiology". Howard B. J., ed., Mosby 1994, 847.

[2] Mroczkowski T. F.: "Sexually transmitted diseases caused by Chlamydia trachomatis". In: "Sexually Transmitted Diseases". Igaku-Shoin, New York, 1990, 1.

[3] Takac, Gorisek B.: "The frequency of Chlamydia trachomatis infection in women with intraepithelial neoplasia of uterine cervix". Eur. J. Gynec. Oncol., 1998, XIX, 5, 492.

[4] Schlott T., Goetz R., Hopper! M. Nagel H., Reimer S., Schumacher- Lutge I. K. et al.: "The in situ polymerase chain reaction for detection of Chlamydia trachomatis". J. Histochemistry and Cytochemistry, 1998, 46, 1017.

[5] David L. M.: "Diagnostic tests and specimens used to screen for Chlamydia trachomatis in genitourinary medicine clinics in the United Kingodom". Int. J. STD-AIDS, 1999, 10, 527.

[6] Natomi T., Ikeda Y., Nagayama A.: "Minimum inhibitory and minimal lethal concentration against Chlamydia trachomatis dependent on time of addition and the duration of the presence of antibiotics". Chemotherapy, 1999, 45, 242.