The relationship between expression of p53/bcl-2 and clinicopathological criteria in cervix squamous cell carcinomas

Objective: Thirty cases with squamous carcinomas of cervix who underwent total abdominal hysterectomy and bilateral salphingo-oopherectomy were reviewed histopathologically.

Materials and methods: The tissues were stained immunohistologically with p53 and bcl-2. Ten cases were selected as well differentiated (large cell keratinizing) carcinoma, ten cases moderately differentiated (large cell non keratinizing) carcinoma and ten cases poorly differentiated carcinoma (includes small cell squamous cell carcinoma).

Results: We observed 11 cases (36.6%) with bcl-2 and 13 cases (43.3%) with p53 positivity. P53 expression showed the opposite correlation to bcl-2 staining.

Conclusion: A decrease in bcl-2 reactivity and an increase in p53 positive staining was noted as the histological differentiation worsened.

p53; Bcl-2; Cervix squamous cell carcmomas

[1] Kromer G.: "The protooncogene bcl-2 and its role in regulating apoptosis". Nat. Med., 1997, 3, 614.

[2] Mc Donnel T. J. et al.: "Importance of the bcl-2 family in cell death regulation". Experientia, 1996, 52, 1008.

[3] Vogelstein B., Kinzler K. W.: "P53 function and dysfunction" Cell, 1993, 70, 523.

[4] Kessis T. D., Slebos R. J., Han S. M. et al.: "P53 gene mutations and MDM2 complification are uncommon in primary carcinomas of the uterine cervix". Am. J. Pathol., 1993, 143, 1398.

[5] Crook T.,W rede D.,T idy J. A.,M ason W. P.,E vans D. J.,V ousden K H.: "Clonal p53 mutation in primary cervical cancer, association with human papilloma virus negative tumors". Lancet, 1992, 239, 1070.

[6] Fujita M.,l nove M.,T anizawa 0.,I wamoto S.,E nomoto T.: "Alterations of p53 gene in human cervical carcinoma with and without human papil Joma virus infection". Cancer Res., 1992, 52, 5323.

[7] Paquette R. C., Lee Y. Y., Wilezynski S. P. et al.: "Mutations of p53 and human papilloma virus infection in cervical carcinoma" Cancer , 1993, 72, 1272.

[8] Busby-Earle R. M., Steel C. M., Williams A. R., Cohen B., Bird C. C.: "P53 mutations in cervical carcinogenesis: low frequency and lock of correlation with human papilloma virus status". Br. J. Cancer, 1994, 69 (4), 732.

[9] Crook T.,V ousden K. H.: "Properties of p53 mutations detected in primary and secondary cervical cancers suggest mechanisms of metastasis and involvement of environment carcinogenesis" EMBO J., 1992, 11, 3935.

[10] Zutter M., Hockenbery D., Silverman G. A., Korsmeyer S. J..: "lmmunolocalisation of the bcl-2 protein within hematopoietic neoplasms". Blood, 1991, 78, 1062.

[11] Doussis I. A.,P ezella F.,L ane D. P. et al.: "An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease". Am. J. Clin. Pathol., 1993, 99, 663.

[12] Lipponen P., Pietlainen T., Kos ma V. M. et al.: "Apoptosis suppressing protein bcl-2 is expression in well differentiated breast carcinomas with favourable prognosis". J. Pathol., 1995, 177, 49.

[13] Haldar S., Negrini M., Monne M. et al.: "Downregulation of bcl-2 by p53 breast cancer cell". Cancer Res., 1994, 54 (8), 2095.

[14] Lisboa B. W., Vogtlander S., Gilster T. et al.: "Molecular and immunohistoche_mical analysis of p53 mutations in sc􀈌apings and tissue from preinvasive and invasive breast cancer". Virchows Arch., 1997, 431, 375.

[15] Lee W. Y., Jin Y. T., Tzeng C. C.: "Reciprocal expression of bcl-2 and p53 in breast ductal carcinoma". Anticancer Res., 1996, 16, 3007.

[16] Konishi I., Fujii S., Nonogaki H., Nanbu Y., lwai T., Mari T.: "lmmunohistochemical analysis of estrogen receptors, progesterone receptors, ki-67 antigen and human papil Joma virus DNA in normal and neoplastic epithelium of the uterine cervix". Cancer, 1991, 68, 1340.

[17] Doglioni C., Dei Tos A. P., Laarino L. et al.: "The prevalence of bcl-2 immunoreactivity in breast carcinomas and its clinicopathological correlates, with particular reference to oestrogen receptor status". Vzrchows Arch., 1994, 424, 47.