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Original Research

Open Access

Granzyme B as a prognostic marker of cervical intraepithelial neoplasia

  • M.C. Kondo1
  • J.C.L. Ribalta1,*,
  • I.D.G. da Silva1
  • M.T.S. Alves2
  • G.R. de Azevedo Focchi2
  • N.V. Martins1
  • J. Focchi1

1Department of Gynecology, UNIFESP (Universidade Federal de Siio Paulo), Brazil

2Department of Pathology, UNIFESP (Universidade Federal de Silo Paulo), Siio Paulo, Brazil

DOI: 10.12892/ejgo20050187 Vol.26,Issue 1,January 2005 pp.87-89

Published: 10 January 2005

*Corresponding Author(s): J.C.L. Ribalta E-mail:

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Abstract

Granzyme B (GrB) is a serine protease synthesized in T lympocytes (CTL), released after T-cell activation resulting from exogenous stimulation. With perforin, GrB discharges apoptotic signals to a target cell and therefore constitutes a marker to identify activated CTL. We aimed to quantify GrB expression by immunohistochemistry staining in 12 tissue fragments of cervical carcinoma, 33 cervical intraepithelial neoplasias treated by LLTEZ and nine cervical pieces without disease. Activated cytotoxic lymphocyte mean values (20 HPF-400x) in both epithelial and stromal pars were 7.11 cells in tissue without neoplasia, 33.45 cells in cervical intraepithelial neoplasia and 139.75 cells in carcinoma samples, with a statistical difference between them. Comparative analysis in the CIN group showed an expressive difference between cases with disease recurrence (19.28 cells) and without recurrence (37.26 cells). Thus, the relation between number of activated CTLs found at the moment of treatment and clinical evolution determined in this study, suggest GrB use as a prognostic marker.

Keywords

Granzyme B; Cervical intraepithelial neoplasia; Prognostic marker; Immunologic marker

Cite and Share

M.C. Kondo,J.C.L. Ribalta,I.D.G. da Silva,M.T.S. Alves,G.R. de Azevedo Focchi,N.V. Martins,J. Focchi. Granzyme B as a prognostic marker of cervical intraepithelial neoplasia. European Journal of Gynaecological Oncology. 2005. 26(1);87-89.

URL:

https://www.ejgo.net/articles/10.12892/ejgo20050187

References

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