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The up-regulation of KCC1 gene expression in cervical cancer cells by IGF-II through the ERK1/2MAPK and PI3K/AKT pathways and its significance

  • S. Zhang 1,*,
  • X. Wu 1
  • T. Jiang 1
  • Y. Lu 1
  • L. Ma 1
  • M. Liang 1
  • X. Sun 1

1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical, University Shenyamg , China

DOI: 10.12892/ejgo20090129 Vol.30,Issue 1,January 2009 pp.29-34

Published: 10 January 2009

*Corresponding Author(s): S. Zhang E-mail: zhangs1@cmu2h.org

Abstract

Objective: To research the alternation effect of insulin-like growth factors-2 (IGF-II) oil the expression of KCl co-transport-1 (KCC1) in the SiHa cells of cervical cancer, and to explore the activation of ERK1/2MAPK and PI3K/AKT signal transduction pathways during the expression process. Method: To apply semi-quantitative RT-PCR and Western blot analysis to measure changes in mRNA and protein expression of KCC1 after exposure to different concentrations of IGF-II for different time durations in the SiHa cells of cervical cancer. The change in protein expression of the ERK1/2 and AKT pathways is also measured. Furthermore, the protein expression variation in ERK1/2, AKT, and KCC1 is observed after the addition of a specific pathway blocker for the ERK1/2MAPK and PI3K/AKT pathways. Results: The mRNA and protein expression of KCC1 increases dramatically after the application of IGF-II on the SiHa cells, and shows a definite dosage-time dependence relationship. The protein phosphorylation is enhanced in the ERK1/2 and AKT pathways, where the protein activity increases. By adding a specific pathway blocker, the protein activity and phosphorylation of the two pathways are no longer promoted even under the effect of IGF-II. Conclusion: IGF-II can enhance KCC1 gene expression in cervical cancer cells through the ERK1/2MAPK and PI3K/AKT signal transduction pathways.

Keywords

Insulin-like growth factor II; KCI Co-transport-1; Cervical cancer; RT-PCR, Western blot.

Cite and Share

S. Zhang ,X. Wu ,T. Jiang ,Y. Lu ,L. Ma ,M. Liang ,X. Sun . The up-regulation of KCC1 gene expression in cervical cancer cells by IGF-II through the ERK1/2MAPK and PI3K/AKT pathways and its significance. European Journal of Gynaecological Oncology. 2009. 30(1);29-34.

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