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Original Research

Open Access

The role of p16INK4a immunostaining in the risk assessment of women with LSIL cytology: a prospective pragmatic study

  • Valasoulis1
  • I. Tsoumpou2,*,
  • C. Founta1
  • M. Kyrgiou3
  • N. Dalkalitsis1
  • M. Nasioutziki4
  • D. Kassanos5
  • E. Paraskevaidis1
  • P. Karakitsos6

1Department of Obstetrics & Gynaecology, University Hospital of Ioannina, Ioannina, Greece

2Department of Obstetrics and Gynaecology, Central Lancashire Teaching Hospitals, Preston, UK

3Department of Obstetrics & Gynaecology, Watford General Hospital, West Hertfordshire Hospitals NHS Trust, London, UK

4Second Department of Obstetrics and Gynaecology, Aristotle University of Thessaloniki, Hippokration Hospital, Thessaloniki, Greece

5Department of Obstetrics and Gynaecology, “Attikon” Hospital, University of Athens, Greece

6Department of Cytopathology, “Attikon” Hospital, University of Athens, Greece

DOI: 10.12892/ejgo201102150 Vol.32,Issue 2,March 2011 pp.150-152

Published: 10 March 2011

*Corresponding Author(s): I. Tsoumpou E-mail: ioantsoumpou@hotmail.com

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Abstract

Background: The detection of high-grade cervical intraepithelial neoplasia (CIN2 or worse) among patients with low-grade cytology (LSIL) is challenging. The aim of this study was to assess the efficacy of p16INK4a in the risk assessment of women with LSIL cytology. Methods: Consecutive liquid-based cytology specimens of 95 LSIL smears were selected and stained for p16INK4a. All patients had colposcopically directed punch biopsies or large loop excision of the transformation zone of the cervix. The endpoint was detection of a biopsy-confirmed CIN2 or worse. Results: The overall sensitivity and specificity of p16INK4a for diagnosis of CIN2+ among LSIL smears were 41% and 86%, respectively. The positive predictive value of the biomarker was 62% and the negative predictive value 72%. Conclusions: The study shows that p16INK4a has low sensitivity but acceptable specificity for evaluation of LSIL smears harbouring high-grade lesions. The marker needs to be further assessed as an adjunct to other tests in an attempt to improve the triage of LSIL cytology smears.

Keywords

p16INK4a; Immunostaining; Cervix; Liquid-based cytology; LSIL; CIN

Cite and Share

Valasoulis,I. Tsoumpou,C. Founta,M. Kyrgiou,N. Dalkalitsis,M. Nasioutziki, D. Kassanos,E. Paraskevaidis,P. Karakitsos. The role of p16INK4a immunostaining in the risk assessment of women with LSIL cytology: a prospective pragmatic study. European Journal of Gynaecological Oncology. 2011. 32(2);150-152.

URL:

https://www.ejgo.net/articles/10.12892/ejgo201102150

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