Twelve-month follow-up detection of high-risk human papillomavirus (HPV) DNA for 93 cases with cervical

intraepithelial neoplasia grade 2 or 3 (CIN 2-3) after a loop electrosurgical excisional procedure (LEEP)

Purpose: The purpose of this study was to follow-up cervical intraepithelial neoplasia grades 2 or 3 (CIN2/3) cases after a loop electrosurgical excisional procedure (LEEP) by liquid-based cytology (LBC) and detection of HPV DNA. Methods: LEEP was performed for the first diagnosed CIN2/3 cases. Six and 12 months after LEEP, LBC and HPV DNA detection were performed. Results: The number of cases with CIN2 accounted for 64.5% (60/93) of the total cases before LEEP. Six months post LEEP, the number of cases with normal LBC and negative HPV DNA accounted for 63.4% (59/93). Cases with abnormal LBC accounted for 17.2 (16/93), and cases with persistent positive HPV DNA accounted for 11.8% (7/93). Two cases had both persistent positive HPV DNA and abnormal LBC. A vaginal intraepithelial neoplasm (VAIN2) was found in one of the HPV DNA persistent positive cases. Twelve months post LEEP, 4.3% (8/93) of the cases were HPV DNA positive. Abnormal LBC was observed in four cases (of which 2 cases were HPV DNA positive) with normal LBC and negative HPV DNA at six months post LEEP. Conclusion: HPV DNA examination is instrumental for the detection of VAIN.

Loop electrosurgical excisional procedure; Human papillomavirus; Follow-up

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