Article Data

  • Views 304
  • Dowloads 127

Original Research

Open Access

Expression of beclin 1, an autophagy-related protein, in human cervical carcinoma and its clinical significance

  • H.Y. Cheng1
  • Y.N. ZHANG2,*,
  • Q.L. Wu3
  • X.M. Sun2
  • J.R. Sun2
  • X. Huang2

1Department of Obstetrics and Gynecology, Qingdao Central Hospital, Qingdao, Shandong, P. R. China

2Department of Gynecology, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong, P. R. China

3Department of Pathology, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong, P. R. China

DOI: 10.12892/ejgo20120115 Vol.33,Issue 1,January 2012 pp.15-20

Published: 10 January 2012

*Corresponding Author(s): Y.N. ZHANG E-mail: zhangyannapds@163.com

Abstract

Purpose: To investigate the impact of beclin 1 on prognosis of cervical cancer, we determined the expression of beclin 1 in cervical cancer, cervical intraepithelial neoplasia (CIN) and normal cervical tissues. Methods: A total of 122 cases of cervical cancer, 35 cases with CIN and 31 cases with uterine fibroids were collected at the Cancer Center of Sun Yat University to determine the expression of beclin I. Results: Beclin l positive rate in normal cervical tissues, CIN tissues and cervical cancers was 83.9%, 74.3% and 53.3%, respectively, and it was significantly different between the three groups (p < 0.01). Beclin 1 expression was negatively correlated with cervical cancer differentiation, lymph node metastasis, recurrence and death (p < 0.05). The negative expression is the risk factor affecting overall survival (p < 0.05) and progression-free survival (PFS) (p < 0.05). Multivariate analysis showed that beclin 1 negative expression was an independent risk factor of PFS time. Conclusions: Beclin I may play a role in the occurrence and development of cervical cancer. Beclin 1 positive expression in patients indicates a better prognosis.

Keywords

Cervical cancer; Autophagy; Beclin 1; Overall survival (OS); Progression-free survival (PFS)

Cite and Share

H.Y. Cheng,Y.N. ZHANG,Q.L. Wu,X.M. Sun,J.R. Sun,X. Huang. Expression of beclin 1, an autophagy-related protein, in human cervical carcinoma and its clinical significance. European Journal of Gynaecological Oncology. 2012. 33(1);15-20.

References

[1] Valdespino V.M., Valdespino V.E.: “Cervical cancer screening: state of the art”. Curr. Opin. Obstet. Gynecol., 2006, 18, 35.

[2] Abraham M.C., Shaham S.: “Death without caspases caspases without death”. Trends Cell. Biol., 2004, 14, 184.

[3] Lockshin R.A., Zakeri Z.: “Apoptosis, autophagy and more”. Int. J. Biochem. Cell. Biol., 2004, 36, 2405.

[4] Shintani T., Klionsky D.J.: “Autophagy in health and disease: a double-edged sword”. Science, 2004, 306, 990.

[5] Yoshimori T.: “Autophagy. a regulated bulk degradation process inside cells”. Biochem. Biophys Res. Commun., 2004, 313, 453.

[6] Levine B., Yuan J.: “Autophagy in cell death: an innocent convict?”. J. Clin. Invest., 2005, 115, 2679.

[7] Aita V.M., Liang X.H., Murty V.V., Pincus D.L., Yu W., Cayanis E. et al.: “Cloning and genomic organization of beclinl a candidate tumor Suppressor gene on chromosome 17q21”. Genomics, 1999, 59, 59.

[8] Liang X.H., Jackson S., Seaman M., Brown K., Kempkes B., Hib-shoosh H. et al.: “Induction of autophagy and inhibition of tumori-genesis by beclin 1”. Natura1, 1999, 402, 672.

[9] Qu X., Yu J., Bhagat G., Furuya N., Hibshoosh H., Troxel A. et al.: “Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene”. J. Clin. Invest., 2003, 112, 1809.

[10] Yue Z., Jin S., Yang C., Levine A.J., Heintz N.: “Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor”. Proc. Natl. Acad. Sci U S A, 2003, 100, 15077.

[11] Miracco C., Cosci E., Oliveri G., Luzi P., Pacenti L., Monciatti I. et al.: “Protein and mRNA expression of autophagy gene Beclin 1 in human brain tumours”. Int. J. Oncol., 2007, 30, 429.[12] Wang Z.H., Xu L., Duan Z.L., Zeng L.Q., Yan N.H., Peng Z.L.: “Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical cancer HeLa cells”. Gynecol. Oncol., 2007, 107, 107.[13] Duan Z.L., Peng Z.L., Wang Z.H.: “Expression and involved signal transduction pathway of autophagy gene beclin 1 in epithelial ovarian cancer”. Sichuan Da Xue Xue Bao Yi Xue Ban, 2007, 38, 239.

[14] Holm E., Hildebrandt W., Kinscherf R., Droge W.: “Low postabsorptive net protein degradation in male cancer patients: lack of sensitivity to regulatory amino acids?”. Oncol. Rep., 2007, 17, 695.[15] Ma D.: “Tumor and tumor metestasis”. In: Zeng Y.X., Lv Y.Y., Zhu M.H. (eds.). Bei Jing, P.R. China, The people’s Hygienic Publication Society, 2003, 242.

[16] Bursch W., Ellinger A., Kienzl H., Torok L., Pandey S., Sikorska M. et al.: “Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells (MCF-7) in culture: the role of autophagy”. Carcinogenesis, 1996, 17, 1595. [17] Scarlatti F., Bauvy C., Ventruti A., Sala G., Cluzeaud F., Vandewalle A. et al.: “Ceramide -mediated macroautophagy involves inhibition of protein kinase B and up-regulation of beclin 1”. J. Biol. Chem., 2004, 279, 18384.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Submission Turnaround Time

Conferences

Top