Establishment of a visualized nude mouse model of cervical carcinoma with high potential of lymph node metastasis via total orthotopic transplantation

Objective: To screen cervical carcinoma (CC) SiHa subgroups with high potential of lymphatic metastasis and establish a visualized nude mouse model of cervical carcinoma with a total orthotopic transplantation approach. Methods: A cervical carcinoma SiHa subgroup with high potential of lymphatic metastasis was isolated by in vitro and in vivo primary culture and continuous passage screening of cervical carcinoma SiHa cells that stably expressed enhanced green fluorescent protein (EGFP). Forty male nude mice aged 6-8 weeks were equally randomized to group A receiving unscreened SiHa/EGFP cells, and group B received in vitro and in vivo screened SiHa/EGFP cells with high potential of lymph node metastasis. Results: In the 20 animals of group A receiving orthotopic transplantation of unscreened SiHa/EGFP cells, the primary tumors were small; local lymph node metastasis was observed in five animals; local organ invasion and distal lymph node metastasis were observed in two animals; and no lung metastasis was observed. In the 20 animals of group B receiving screened SiHa/EGFP cells, local lymph node metastasis occurred in all animals; distal lymph node metastasis was observed in 18 animals; and lung metastasis was observed in seven animals. Conclusion: A cervical carcinoma SiHa subgroup with high potential of lymphatic metastasis was isolated and a visualized nude mouse model of cervical carcinoma with high potential of lymph node metastasis was established through total orthotopic transplantation successfully. This provided a good platform for the further study of cervical carcinoma-related mechanisms, especially mechanisms of lymphatic metastasis in cervical carcinoma.

Cervical carcinoma; Orthotopic transplantation; Lymphatic metastasis; Visualized animal model; Enhanced green fluorescentprotein (EGFP)

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