Article Data

  • Views 373
  • Dowloads 120

Original Research

Open Access

Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer

  • Y.X. Cheng1
  • Q.F. Zhang1
  • F. Pan2
  • J.L. Huang1
  • B.L. Li1
  • M. Hu1
  • M.Q. Li1
  • Ch. Chen1,*,

1Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China

2Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China

3Department of Breast and Thyriod Surgery, Renmin Hospital of Wuhan University, Wuhan, China

DOI: 10.12892/ejgo2845.2016 Vol.37,Issue 2,April 2016 pp.238-243

Published: 10 April 2016

*Corresponding Author(s): Ch. Chen E-mail:


Objectives: To investigate the effect and the mechanism of anti-tumor agent hydroxycamptothecin (HCPT) on HeLa cells in cervical cancer. Materials and Methods: Autophagy and apoptosis were detected by western blotting and the transfection of GFP-LC3 shRNA as well as Hoechst staining. Results: The authors found that the expression of the regulators of Beclin-1, p62, and microtubule-associated protein 1 light chain 3 (LC3) upregulated and then triggered the occurrence of cell autophagy. On the other hand, HCPT could induce to the formation of autophagy and resulted in cell apoptosis after autophagy. Conclusion: HCPT can alter cell autophagy and then trigger cell apoptosis to achieve antitumor effects.


HCPT; Cervical cancer; Autophagy; Apoptosis.

Cite and Share

Y.X. Cheng,Q.F. Zhang,F. Pan,J.L. Huang,B.L. Li,M. Hu,M.Q. Li,Ch. Chen. Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer. European Journal of Gynaecological Oncology. 2016. 37(2);238-243.


[1] Karimi Z.M., Behtash N., Chiti Z., Kargar S.: “Cervial cancer and HPV vaccines in developing countries”. Asian Pac. J. Cancer Prev., 2009, 10, 969.

[2] Quinn M.A., Benedet J.L., Odicino F., Maisonneuve P., Beller U., Creasman W.T., et al.: “Carcinoma of the cervix utcri. FIGO 26th annual report on the results of treatment in gynecological cancer”. Int. J. Gynaecol. Obstet., 2006, 95, S43.

[3] Croswell J., Costello A.: “Screening for cervical cancer”. Am. Fam. Physician, 2012, 86, 563.

[4] Levine B., Deretic V.: “Unveiling the roles of autophagy in innate and adaptive immunity”. Nat. Rev. Immunol., 2007, 7, 767.

[5] Shintani T., Klionsky D.J.: “Autophagy in health and disease: a double-edged sword”. Science, 2004, 306, 990.

[6] Kuballa P., Nolte W.M., Castoreno A.B., Xavier R.J.: “Autophagy and the immnune system”. Annu. Rev. Immunol., 2012, 30, 611.

[7] Kondo Y., Kondo S.: “Autophagyand cancer therapy”. Autophagy, 2006, 2, 85.

[8] Hippert M.M., O’Toole P.S., Thorbum A.: “Autophagy in cancer: good, bad, or both? ”. Cancer Res., 2006, 66, 9349.

[9] Kanzawa T., Germano I.M., Komata T., Ito H., Kondo Y., Kondo S.: “Role of autophagy in temozolomide- induced cytotoxicity for malignant glioma cells”. Cell Death Differ., 2004, 11, 448.

[10] Lee S., Lee M.S., Baek M., Lee D.Y., BangY.J., Cho H.N., et al.: “MAPK signaling is involved in camptothecin- Induced cell death”. Mol. Cells., 2002, 14, 348.

[11] Kang M.R., Muller M.T., Chung I.K.: “Telomeric DNA damage by topoisomerase I. A possible mechanism for cell killing by camptothecin”. J. Biol. Chem., 2004, 279, 12535.

[12] Liao Z., Robey R.W., Guirouilh-Barbat J., To K.K., Polgar O., Susan E., et al.: “Reduced Expression of DNA Topoisomerase I in SF295 human glioblastoma cells selected for resistance to homocamptothecin and diflomotecan”. Mol. Pharmacol., 2008, 73, 490.

[13] Hu W., Zhang C., Fang Y., Lou C.: “Anticancer properties of 10-Hydroxycamptothecin in a murine melanoma pulmonary metastasis model in vitro and in vivo”. Toxicol In Vitro, 2011, 25, 513.

[14] Mattern M.R., Hofmann G.A., Mccabe F.L., Johnson R.K.: “Synergistic cell killing by ionizing radiation and topoisomerase I in hibitor topotecan”. Cancer Res., 1991, 51, 5813.

[15] Klionsky D.J., Emr S.D.: “Autophagy as a regulated pathway of cellular degradation”. Science, 2000, 290, 1717.

[16] Han C., Sun B., Wang W.,Cai W., Lou D., Sun Y., et al.: “Overexpression of microtubule-associated protein-1 light chain 3 is associated with melanoma metastasis and vasculogenic mimicry”. Tohoku J. Exp. Med., 2011, 223, 243.

[17] Aita V.M., Liang X.H., Murty V.V.,Pincus D.L., Yu w., Cayanis E., et al.: “Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21”. Genomics, 1999, 59, 59.

[18] Qu X., Yu J., Bhagat G., Furuya N., Hibshoosh H., Troxel A., et al.: “Promotion of tumorigenesis by heterozygous disruption of the be-clin l autophagy gene”. J. Clin. Invest., 2003, 112, 1809.

[19] Miracco C., Cosci E., Oliveri G., Luzi P., Pacenti L., Monciatti I., et al.: “Protein and mRNA expression of autophagy gene Beclin 1 in human brain tumours”. Int. J. Oncol., 2007, 30, 429.

[20] Wang Z.H., Xu L., Duan Z.L., Zeng L.Q., Yan N.H.,Peng Z.L.:”Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical HeLa cells”. Gynecol. Oncol. 2007, 107, 107.

[21] Duan Z.L., Peng Z.L., Wang Z.H.:. “Expression and involved signal transduction pathway of autophagy gene Beclin 1 in epithelial ovarian cancer”. J. Sichuan Univ. (Med. Sci. Edi.), 2007, 38, 239.

[22] Yue Z., Jin S., Yang C., Levine A., Heintz N.: “Beclin l, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor”. Proc. Natl. Acad. Sci. U S A, 2003, 100, 15077.

[23] Zanghong W., Zhilan P., Zhenling D., Hui L.: ”Role of endoplasmic reticulum stress-autophagic response in cisplatin-induced cervical cancer HeLa cells death”. J. Sichuan Univ. (Med. Sci. Edi.), 2006, 37, 860.

[24] Pandey S., Chandravati.: “Autophagy in cervical cancer: an emerging therapeutic target”. Asian Pacific J. Cancer Prev., 2012, 13, 4867.

[25] Kung C.P., Budina A., Balaburski G., Bergenstock M.K., Murphy M.: “Autophagy in tumor suppression and cancer therapy”. Crit. Rev. Eukaryot. Gene Expr., 2011, 21, 71.

[26] Lee J., Giordano S., Zhang J.: “Autophagy, mitochondria and oxidative stress: cross-talk and redox signaling”. Biochem J., 2012, 441, 523.

[27] Zanghong W.M., Jing Z., Tao B.:” Role of endoplasmic reticulum stress- autophagicresponse in cisplatin-induced cervical cancer HeLa cells death”. J. Shansi Med. Univ., 2012, 43, 808.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Submission Turnaround Time