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Original Research

Open Access

Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer

  • Y.X. Cheng1
  • Q.F. Zhang1
  • F. Pan2
  • J.L. Huang1
  • B.L. Li1
  • M. Hu1
  • M.Q. Li1
  • Ch. Chen1,*,

1Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China

2Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China

3Department of Breast and Thyriod Surgery, Renmin Hospital of Wuhan University, Wuhan, China

DOI: 10.12892/ejgo2845.2016 Vol.37,Issue 2,April 2016 pp.238-243

Published: 10 April 2016

*Corresponding Author(s): Ch. Chen E-mail: 1207586302@qq.com

Abstract

Objectives: To investigate the effect and the mechanism of anti-tumor agent hydroxycamptothecin (HCPT) on HeLa cells in cervical cancer. Materials and Methods: Autophagy and apoptosis were detected by western blotting and the transfection of GFP-LC3 shRNA as well as Hoechst staining. Results: The authors found that the expression of the regulators of Beclin-1, p62, and microtubule-associated protein 1 light chain 3 (LC3) upregulated and then triggered the occurrence of cell autophagy. On the other hand, HCPT could induce to the formation of autophagy and resulted in cell apoptosis after autophagy. Conclusion: HCPT can alter cell autophagy and then trigger cell apoptosis to achieve antitumor effects.


Keywords

HCPT; Cervical cancer; Autophagy; Apoptosis.

Cite and Share

Y.X. Cheng,Q.F. Zhang,F. Pan,J.L. Huang,B.L. Li,M. Hu,M.Q. Li,Ch. Chen. Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer. European Journal of Gynaecological Oncology. 2016. 37(2);238-243.

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