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Original Research

Open Access

Differential gene expression profile in cervical cancer and parenchyma infected with human papillomavirus 16 screened by cDNA microarray

  • Y. L. Wang1,2
  • X. P. Ding1,*,
  • Z. R. Xiong3
  • H. Liao2

1Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu

2People’s Hospital of Pengzhou, Chengdu

3Gene Company Limited, Chengdu (China)

DOI: 10.12892/ejgo340205 Vol.34,Issue 2,March 2013 pp.132-137

Published: 24 March 2013

*Corresponding Author(s): X. P. Ding E-mail:


Purpose of investigation: The object of this study was to observe differential gene expression profiles related to human papillomavirus (HPV) associated with postoperative cervical cancer. Materials and Methods: Three women with cervical cancer and infected with HPV 16 were selected by a genotyping method. Samples of tissue were collected from the tumor center (T), from the adjacent tumor (N), and from tissues distal to the tumors (F). Human gene expression in cervical cancers and parenchyma and normal cervical tissues was analysed using a gene expression system. Results: Comparison of T vs F samples revealed 673 differentially-expressed genes and comparison of N vs F samples produced 56 differentially-expressed genes. These genes were related to signal transduction, metabolism, immunity response, protein biosynthesis, and expressed sequences tags (ESTs). Conclusion: Therefore the authors conclude that differences in gene expression could be seen among cervical cancers and the parenchyma and the normal cervical tissues.


Cervical carcinoma; Human papillomavirus; Gene expression; Gene microarray.

Cite and Share

Y. L. Wang,X. P. Ding,Z. R. Xiong,H. Liao. Differential gene expression profile in cervical cancer and parenchyma infected with human papillomavirus 16 screened by cDNA microarray. European Journal of Gynaecological Oncology. 2013. 34(2);132-137.


[1] Parkin M., Bray F.: “The burden of HPV-related cancers”. Vaccine, 2006, 24, 11.

[2] Parkin D.M.: “Global cancer statistics in the year 2000”. Lancet Oncol., 2001, 2, 533.

[3] Parkin D.M., Bray F., Feriay J., Pisani P.: “Estimating the world cancer burden: Globocan 2000”. Int. J. Cancer, 2001, 94, 153.

[4] Song S.H., Lee J.K., Oh M.J., Hur J.Y., Park Y.K., Saw H.S.: “Risk factors for the progression or persistence of untreated mild dysplasia of the uterine cervix”. Int. J. Gynecol. Cancer, 2006, 16, 1608.

[5] Vinokurova S., Wentzensen N., Kraus I., Klaes R., Driesch C., Melsheimer P. et al.: “Type-dependent integration frequency of human papillomavirus genomes in cervical lesions”. Cancer Res., 2008, 68, 307.

[6] Muñoz N., Bosch F.X., de Sanjosé S., Herrero R., Castellsagué X., Shah K.V. et al.: “Epidemiologic classification of human papillomavirus types associated with cervical cancer”. N. Engl. J. Med., 2003, 348, 518.

[7] Dell G., Gaston K.: “Human papillomaviruses and their role in cervical cancer”. Cell. Mol. Life Sci., 2001, 58, 1923.

[8] Wiley D., Masongsong E.: “Human papillomaviruses: the burden of infection”. Obstet. Gynecol. Surv., 2006, 61, 13.

[9] Tusher V., Tibshirani R., Chu G.: “Significance analysis of microarrays applied to transcriptional responses to ionizing radiation”. Proc. Natl. Acad. Sci. USA, 2001, 98, 5116.

[10] Chun-Ling Zhao, Li-Mei Chen, Zhi-Qin Gao, Chang-Qing Du, Zhi-Fang Pan, Wen-Jing Yu: “Clinical significance of cyclin B1 and cyclin-dependent kinase 1 expression in human esophageal squamous cell carcinoma”. World Chin. J. Digestol., 2009, 17, 2374.

[11] Tian Qi, Sheng-jun L.V.: “Expression and significance of Survivin,Ki-67 in cervical carcinoma and ervical intraepithelial neoplasia”. Chin. J. Lab. Diagn., 2011, 12, 2067.

[12] Kjaer S.K., van den Brule A.J., Paull G., Svare E.I., Sherman M.E., Thomsen B.L. et al.: “Types specific persistence of high risk human papillomavirus (HPV) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow-up study”. BMJ, 2002, 325, 572.

[13] Rong Ye-fei, Xu Bin, Jin Da-yong: “The progress of MUC1 in the research of tumor development, diagnosis and therapy”. Fund. Univ. J. Med. Sci., 2008, 35, 156.

[14] Arias-Pulido H., Peyton C.L., Joste N.E., Vargas H., Wheeler C.M.: “Human papillomavirus type 16 integration in cervical carcinoma in situ and in invasive cervical cancer”. Clin. Microbiol., 2006, 44, 1755.

[15] Boccardo E.: “HPV-mediated genome instability: at the roots of cervical carcinogenesis”. Cytogenet. Genome Res., 2010, 128, 57.

[16] Tao W.U., Zou X.M., Song M.L., Li M.Q.: “Expression of TβR-II, Smad4 and Smad7 protein in human gastric carcinoma”. J. Pract. Oncol., 2007, 21, 513.

[17] Xueyan Yang, Binghong Xiong, Li M.A.: “P16, P15 and PCNA expression in cervical cancer and its clinical significance”. Med. Theory Pract., 2010, 23, 913.

[18] Yu X.P., Huang L.M., Huang Y.L., Song Y.H., You C.C.: “Expression of P16, P15, Smad7 and TGF-β1 in cervical cancer and the significance”. Mater. Child Health Care of China, 2011, 26, 2813.

[19] Steeg P.S.: “Tumor metastasis: mechanistic insights and clinical challenges”. Nat. Med., 2006, 12, 895.

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