An experimental study of cyclinD1 expression in human epithelial ovarian cancer cells

Purpose of investigation: As an important oncogene, cyclinD1 has close relationship with carcinomars. This study aims to investigate the correlation between cyclinD1 expression and ovarian cancer cell growth capability and apoptosis. Materials and Methods: Epithelial ovarian cancer (EOC) cell line 3AO was cultured and incubated with different concentrations (0, 1, 5, and 10 ug/ml) of cisplatin. RT-PCR and FCM analysis were used to detect cyclinD1 mRNA and protein expression, 3AO cell cycles, cell growth capability, and apoptosis were assessed by FCM. Results: EOC cell 3AO presented anti-apoptosis capabilities and cyclinD1 overexpression. After treatment with cisplatin, low cyclinD1 expression, decelerated cell growth capability, and high cell apoptosis levels were detected. Conclusion: CyclinD1 expression has closely correlated with EOC cell growth and apoptosis. CyclinD1 may be a good biomarker for monitoring the progress of EOC.

Epithelial ovarian cancer; CyclinD1; RT- PCR; Flow cytometry.

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