The effect of RNAi silencing Tspan5 expression on the proliferation and migration of human choriocarcinoma cell line JEG-3

Objective: To study the effect of Tspan5 on the proliferation and migration of trophoblast cell, seeking a new target for the treatment of gestational trophoblastic disease. Materials and Methods: The authors used RNAi to silence the Tspan5 expression in human choriocarcinoma cell line JEG-3 first, and then used Western blotting to detect the expression of Tspan5, calculating the efficiency of interference. CCK-8 test, colony formation, and scratch assay were used to detect the proliferation and migration of the before and after interference JEG-3 cells. Results: Western blotting results showed that Tspan5/NC band gray value was highest and Tspan5/1005 lowest, so the authors chose Tspan5/1005 as an interference group to finish the follow-up experiment. Interference group (Tspan5/1005) clone number of JEG-3 cell was significantly reduced compared with the control group (Tspan5/NC) (148±23 vs. 276±31, p < 0.01). There was no significant difference between blank group and control group (328±39 vs. 276±31, p > 0.1). The JEG-3 cell proliferation activity of blank group was the highest, the control group (Tspan5/NC) group’s proliferation activity was slightly lower, however there was no significant difference (1.200 ± 0.103 vs. 1.185 ± 0.022, p > 0.05) between the two. Compared with the control group (Tspan5/NC), the JEG-3 cell proliferation activity of interference group (Tspan5/1005) decreased significantly (1.185 ± 0.022 vs. 1.076 ±0.028, p < 0.01). In the absolute value of the scratch width, 24 hours after scratching minus the scratch width 0 hours after scratching, the interference group (Tspan5/1005) was significantly less than the control group (Tspan5/NC) (1.27 ± 0.03 cm vs. 1.97 ± 0.38 cm, p < 0.05). Conclusion: In this study, the authors found that the proliferation and migration of choriocarcinoma cells were reduced after using RNAi technology to knockdown Tspan5 expression. The quantity of Tspan5 expression was positively related with proliferation and migration of choriocarcinoma cells.

Tspan5; Human choriocarcinoma cell line JEG-3; Proliferation; Migration; Transmembrane 4 superfamily; RNA interference.

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