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Original Research

Open Access

RAPH1 in cervical cancer proliferation, migration, and invasion: in vitro studies with SiHa and HeLa cells

  • H.-F. Chen1,†
  • P. Yang1,†
  • G.-Q. Yang1
  • Y. Lin2
  • J.-K. Li1,2,*,

1Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China

2Key Laboratory of Birth Defects and Related Disease of Women and Children (Sichuan University), Ministry of Education, Chengdu, China

DOI: 10.12892/ejgo4529.2019 Vol.40,Issue 2,April 2019 pp.342-344

Accepted: 10 November 2017

Published: 10 April 2019

*Corresponding Author(s): J.-K. Li E-mail:

† These authors contributed equally.


Background: Ras association and pleckstrin homology domains 1 (RAPH1) may play a critical role in cervical cancer development, prompting us to investigate its potential role(s) in proliferation, migration, and invasion by cervical cancer cells. Materials and Methods: RAPH1 was knocked down in SiHa and HeLa cervical cancer cells using short interfering RNA. Effects of RAPH1 knock-down were assessed on cell proliferation using the CCK8 assay, and on cell migration and invasion using in vitro transwell assays. Results: Efficient RAPH1 knock-down significant reduced the proliferation, migration, and invasion abilities of SiHa and HeLa cells (p < 0.05). Conclusions: RAPH1 may promote tumor cell proliferation, migration, and invasion during cervical cancer development.


RAPH1; Cervical cancer; Cell migration

Cite and Share

H.-F. Chen,P. Yang,G.-Q. Yang,Y. Lin,J.-K. Li. RAPH1 in cervical cancer proliferation, migration, and invasion: in vitro studies with SiHa and HeLa cells. European Journal of Gynaecological Oncology. 2019. 40(2);342-344.


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