Long non-coding RNA BANCR contributes to cervical adenocarcinoma migration by affecting epithelial-mesenchymal transition
1Department of Obstetrics and Gynecology, Clinical Medical College of Yangzhou University, Yangzhou (China)
DOI: 10.12892/ejgo4734.2019 Vol.40,Issue 3,June 2019 pp.408-412
Published: 10 June 2019
Purpose of Investigation: The BRAF-activated non-coding RNA (BANCR) functions are both an oncogene and a tumor suppressor; however, little is known about the role of BANCR in the development of cervical adenocarcinoma. Materials and Methods: The authors investigated BANCR’s role in cervical adenocarcinoma by assessing BANCR levels in cervical adenocarcinoma and matched adjacent normal tissues from nine patients using qRT-PCR. They also used lentiviral vectors to establish cervical adenocarcinoma cell lines to investigate the effects of BANCR knockdown on cancer cell proliferation, apoptosis, and migration. Results: RT-PCR results showed that BANCR was frequently overexpressed in cancer tissues and cervical adenocarcinoma Hela cells. Knockdown of BANCR inhibited the migration of Hela cells in vitro. Further investigation into the mechanisms responsible for the migration effects revealed that BANCR induced the epithelial-mesenchymal transition (EMT) in Hela cells. Conclusion: These results revealed the important role of BANCR in the molecular etiology of cervical adenocarcinoma and implied the potential application of BANCR in cervical adenocarcinoma therapy.
Cervical adenocarcinoma; Long non-coding RNA; BANCR; migration; EMT.
Q. Guo,Y. Huang,J. Xu,W. Wang,J. Gao,Y. Su,Y. Gu,X. Yin. Long non-coding RNA BANCR contributes to cervical adenocarcinoma migration by affecting epithelial-mesenchymal transition. European Journal of Gynaecological Oncology. 2019. 40(3);408-412.
 Adamson P.C., Huchko M.J., Moss A.M., Kinkel H.F., MedinaMarino A.: “Acceptability and Accuracy of Cervical Cancer Screening Using a Self-Collected Tampon for HPV Messenger-RNA Testing among HIV-Infected Women in South Africa”. PLoS One, 2015, 10, e0137299.
 Takeuchi S.: “Biology and treatment of cervical adenocarcinoma”. Chin. J. Cancer Res., 2016, 28, 254.
 Roma A.A.: “Patterns of Invasion of Cervical Adenocarcinoma as Predicators of Outcome”. Adv. Anat. Pathol., 2015, 22, 345.
 Punt S., van Vliet M.E., Spaans V.M., de Kroon C.D., Fleuren G.J., Gorter A., Jordanova ES.: “FoxP3(+) and IL-17(+) cells are correlated with improved prognosis in cervical adenocarcinoma”. Cancer Immunol. Immunother., 2015, 64, 745.
 Ponting C.P., Oliver P.L., Reik W.: “Evolution and functions of long noncoding RNAs”. Cell, 2009, 136, 629.
 Li J., Tian H., Yang J., Gong Z.: “Long Noncoding RNAs Regulate Cell Growth, Proliferation, and Apoptosis”. DNA Cell Biol., 2016, 35, 459.
 Schmitt AM., Chang H.Y.: “Long Noncoding RNAs in Cancer Pathways”. Cancer Cell, 2016, 29, 452.
 Liu Y., Zheng L., Wang Q., Hu YW.: “Emerging roles and mechanisms of long noncoding RNAs in atherosclerosis”. Int. J. Cardiol., 2017, 228, 570.
 Liu J., Cheng G., Yang H., Deng X., Qin C., Hua L., et al.: “Reciprocal regulation of long noncoding RNAs THBS4003 and THBS4 control migration and invasion in prostate cancer cell lines”. Mol. Med. Rep., 2016, 14, 1451.
 Shi T., Gao G., Cao Y.: “Long Noncoding RNAs as Novel Biomarkers Have a Promising Future in Cancer Diagnostics”. Dis. Markers, 2016, 2016, 9085195.
 Yang Z., Guo X., Li G., Shi Y., Li L.: “Long noncoding RNAs as potential biomarkers in gastric cancer: Opportunities and challenges”. Cancer Lett., 2016, 371, 62.
 Flockhart R.J., Webster D.E., Qu K., Mascarenhas N., Kovalski J., Kretz M., et al.: “BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration”. Genome Res., 2012, 22, 1006.
 Li L., Zhang L., Zhang Y., Zhou F.: “Increased expression of LncRNA BANCR is associated with clinical progression and poor prognosis in gastric cancer”. Biomed. Pharmacother., 2015, 72, 109.
 Guo Q., Zhao Y., Chen J., Hu J., Wang S., Zhang D., et al.: “BRAFactivated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition”. Oncol. Lett., 2014, 8, 869.
 Wang Y., Guo Q., Zhao Y., Chen J., Wang S., Hu J., et al.: “BRAFactivated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma”. Oncol. Lett., 2014, 8, 1947.
 Gurzu S., Turdean S., Kovecsi A., Contac AO., Jung I.: “Epithelialmesenchymal, mesenchymal-epithelial, and endothelial-mesenchymal transitions in malignant tumors: An update”. World J. Clin. Cases, 2015, 3, 393.
 Ren Y., Lu H., Zhao D., Ou Y., Yu K., Gu J., et al.: “LTPB2 acts as a prognostic factor and promotes progression of cervical adenocarcinoma”. Am. J. Transl. Res., 2015, 7, 1095.
 Zhang D., Sun G., Zhang H., Tian J., Li Y.: “Long non-coding RNA ANRIL indicates a poor prognosis of cervical cancer and promotes carcinogenesis via PI3K/Akt pathways”. Biomed. Pharmacother., 2017, 85, 511.
 Cao S., Liu W., Li F., Zhao W., Qin C.: “Decreased expression of lncRNA GAS5 predicts a poor prognosis in cervical cancer”. Int. J. Clin. Exp. Pathol., 2014, 7, 6776.
 Zhang J., Lin Z., Gao Y., Yao T.: “Downregulation of long noncoding RNA MEG3 is associated with poor prognosis and promoter hypermethylation in cervical cancer”. J. Exp. Clin. Cancer Res., 2017, 36, 5.
 Ying L., Chen Q., Wang Y., Zhou Z., Huang Y., Qiu F.: “Upregulated MALAT-1 contributes to bladder cancer cell migration by inducing epithelial-to-mesenchymal transition”. Mol. Biosyst., 2012, 8, 2289.
 Luo M., Li Z., Wang W., Zeng Y., Liu Z., Qiu J.: “Long non-coding RNA H19 increases bladder cancer metastasis by associating with EZH2 and inhibiting E-cadherin expression”. Cancer Lett., 2013, 333, 213.
 Xu Z.Y., Yu Q.M., Du Y.A., Yang L.T., Dong R.Z., Huang L., et al.: “Knockdown of long non-coding RNA HOTAIR suppresses tumor invasion and reverses epithelial-mesenchymal transition in gastric cancer”. Int. J. Biol. Sci., 2013, 9, 587.
Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.
Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.
Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.
JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.
Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.
BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.
Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.