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Original Research

Open Access

DNAJB5 (hsc40) gene as a novel biomarker for cervical cancer

  • Hu Yu1,2,3
  • Ying Chen1,2,3
  • Guoxin Zhang4
  • Huijuan Wu1,2,3
  • Ke Wang1,2,3,*,

1Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute & Hospital, 30060 Tianjin, China

2Tianjin Clinical Research Center for Cancer, 30060 Tianjin, China

3Department of Gynaecological Oncology, Tianjin Medical University Cancer Institute and Hospital, 300060 Tianjin, China

4Department of respiratory and critical care medicine, Tianjin Chest Hospital, 300051 Tianjin, China

DOI: 10.22514/ejgo.2022.023

Submitted: 17 May 2022 Accepted: 13 June 2022

Online publish date: 07 July 2022

*Corresponding Author(s): Ke Wang E-mail: jonshon20031013@gmail.com

Abstract

Cervical cancer is among the most common malignant tumor and leading cause of cancer-related mortality in women worldwide. Heat shock proteins (HSPs) are reported increasingly as potential biomarkers and drug targets for cancers. DNAJB5 has been shown to be involved in HSP binding and response to unfolded proteins, but its role in cervical cancer remains currently unclear. In this study, DNAJB5 was knocked down in SiHa cells by short hairpin RNA (shRNA) and verified by quantitative real-time polymerase chain reaction (qRT-PCR) as well as Western blotting. Its effects on cell proliferation, apoptosis, viability, and cell cycle were investigated by Celigo assay, Annexin V-Allophycocyanin (Annexin V-APC) assay, tetrazolium [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] dye (MTT) assay and propidium iodide (PI) staining assay, respectively. shRNA experiments successfully knocked down DNAJB5 expression, which decreased the proliferation of SiHa cells, reduced the number of viable cells, affected the cell cycle of SiHa cells and increased their apoptosis. The knockdown of DNAJB5 acted as a tumor suppressor in cervical cancer cells, suggesting DNAJB5 as a new biomarker candidate and potential target for cervical cancer treatment.


Keywords

DNAJB5; Cervical cancer; shRNA; HSP


Cite and Share

Hu Yu,Ying Chen,Guoxin Zhang,Huijuan Wu,Ke Wang. DNAJB5 (hsc40) gene as a novel biomarker for cervical cancer. European Journal of Gynaecological Oncology. 2022.doi:10.22514/ejgo.2022.023.

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