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Original Research

Open Access

Knockdown of GALNT7 promotes cell apoptosis and autophagy of breast cancer cells by inactivation of STAT3

  • Jiao Xue1
  • Qiang Yu2,*,

1Department of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, 213000 Changzhou, Jiangsu, China

2Department of General Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, 213000 Changzhou, Jiangsu Province, China

DOI: 10.22514/ejgo.2022.035 Vol.43,Issue 4,August 2022 pp.79-85

Submitted: 13 May 2022 Accepted: 06 July 2022

Published: 15 August 2022

*Corresponding Author(s): Qiang Yu E-mail: qiang_yu165@163.com

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Abstract

Breast cancer, a most common malignancy among women with high lethality. Polypeptide-N-acetyl-galactosaminlytransferase 7 (GALNT7) contributes to carcino-genesis and development of breast cancer. Activation of signal transducer and activator of transcription 3 (STAT3) is vital in the initiation and development of cancers. In this work, GALNT7 was upregulated in breast cancer cells, and promoted cell proliferation. GALNT7 knockdown suppressed proliferation of breast cancer cells. GALNT7 overexpression upregulated B-cell lymphoma protein 2 (BCL2) expression and suppressed the expression levels of Bcl-2-associated X protein (BAX), cleaved caspase 3, LC3-II/LC3-I ratio and Atg5, which was dramatically suppressed by knockdown of GALNT7 in breast cancer cells. GALNT7 overexpression favored the phosphorylation of STAT3, which was significantly prevented by knockdown of GALNT7 in breast cancer cells. Inactivation of STAT3 by Stattic increased the expressions levels of LC3-II/LC3-I ratio and Atg5, which was prevented by GALNT7 overexpression in breast cancer cells. Knockdown of GALNT7 promoted cell apoptosis and autophagy of breast cancer cells via inactivation of STAT3.


Keywords

GALNT7; STAT3; Breast cancer; Apoptosis; Autophagy


Cite and Share

Jiao Xue,Qiang Yu. Knockdown of GALNT7 promotes cell apoptosis and autophagy of breast cancer cells by inactivation of STAT3. European Journal of Gynaecological Oncology. 2022. 43(4);79-85.

URL:

https://www.ejgo.net/articles/10.22514/ejgo.2022.035

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