Knockdown of GALNT7 promotes cell apoptosis and autophagy of breast cancer cells by inactivation of STAT3
1Department of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, 213000 Changzhou, Jiangsu, China
2Department of General Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, 213000 Changzhou, Jiangsu Province, China
DOI: 10.22514/ejgo.2022.035 Vol.43,Issue 4,August 2022 pp.79-85
Submitted: 13 May 2022 Accepted: 06 July 2022
Published: 15 August 2022
*Corresponding Author(s): Qiang Yu E-mail: email@example.com
Breast cancer, a most common malignancy among women with high lethality. Polypeptide-N-acetyl-galactosaminlytransferase 7 (GALNT7) contributes to carcino-genesis and development of breast cancer. Activation of signal transducer and activator of transcription 3 (STAT3) is vital in the initiation and development of cancers. In this work, GALNT7 was upregulated in breast cancer cells, and promoted cell proliferation. GALNT7 knockdown suppressed proliferation of breast cancer cells. GALNT7 overexpression upregulated B-cell lymphoma protein 2 (BCL2) expression and suppressed the expression levels of Bcl-2-associated X protein (BAX), cleaved caspase 3, LC3-II/LC3-I ratio and Atg5, which was dramatically suppressed by knockdown of GALNT7 in breast cancer cells. GALNT7 overexpression favored the phosphorylation of STAT3, which was significantly prevented by knockdown of GALNT7 in breast cancer cells. Inactivation of STAT3 by Stattic increased the expressions levels of LC3-II/LC3-I ratio and Atg5, which was prevented by GALNT7 overexpression in breast cancer cells. Knockdown of GALNT7 promoted cell apoptosis and autophagy of breast cancer cells via inactivation of STAT3.
GALNT7; STAT3; Breast cancer; Apoptosis; Autophagy
Jiao Xue,Qiang Yu. Knockdown of GALNT7 promotes cell apoptosis and autophagy of breast cancer cells by inactivation of STAT3. European Journal of Gynaecological Oncology. 2022. 43(4);79-85.
 Thorat MA, Balasubramanian R. Breast cancer prevention in high-risk women. Best Practice & Research Clinical Obstetrics & Gynaecology. 2020; 65: 18–31.
 Solanki M, Visscher D. Pathology of breast cancer in the last half century. Human Pathology. 2020; 95: 137–148.
 Song P, Li Y, Dong Y, Liang Y, Qu H, Qi D, et al. Estrogen receptor β inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy. Journal of Experimental & Clinical Cancer Research. 2019; 38: 354.
 Kim M, Kim H, Kim J, Kim E, Lee B, Lee SY, et al. Chaenomelis fructus inhibits osteoclast differentiation by suppressing NFATc1 expression and prevents ovariectomy-induced osteoporosis. BMC Complementary Medicine and Therapies. 2020; 20: 35.
 Wu H, Chen J, Li D, Liu X, Li L, Wang K. MicroRNA-30e functions as a tumor suppressor in cervical carcinoma cells through targeting GALNT7. Translational Oncology. 2017; 10: 876–885.
 Cao Q, Wang N, Ren L, Tian J, Yang S, Cheng H. MiR-125a-5p post-transcriptionally suppresses GALNT7 to inhibit proliferation and invasion in cervical cancer cells via the EGFR/PI3K/AKT pathway. Cancer Cell International. 2020; 20: 117.
 Lu Q, Xu L, Li C, Yuan Y, Huang S, Chen H. MiR-214 inhibits invasion and migration via downregulating GALNT7 in esophageal squamous cell cancer. Tumor Biology. 2016; 37: 14605–14614.
 Shan SW, Fang L, Shatseva T, Rutnam ZJ, Yang X, Du W, et al. Mature miR-17-5p and passenger miR-17-3p induce hepatocellular carcinoma by targeting PTEN, GalNT7 and vimentin in different signal pathways. Journal of Cell Science. 2013; 126: 1517–1530.
 Tella SH, Gallagher JC. Prevention and treatment of postmenopausal osteoporosis. The Journal of Steroid Biochemistry and Molecular Biology. 2014; 142: 155–170.
 Hua S, Li H, Liu Y, Zhang J, Cheng Y, Dai C. High expression of GALNT7 promotes invasion and proliferation of glioma cells. Oncology Letters. 2018; 16: 6307–6314.
 Chen J, Wu P, Xia R, Yang J, Huo X, Gu D, et al. STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy. Molecular Cancer. 2018; 17: 6.
 Liu K, Ren T, Huang Y, Sun K, Bao X, Wang S, et al. Apatinib promotes autophagy and apoptosis through VEGFR2/STAT3/BCL-2 signaling in osteosarcoma. Cell Death & Disease. 2017; 8: e3015–e3015.
 Banerjee K, Resat H. Constitutive activation of STAT3 in breast cancer cells: a review. International Journal of Cancer. 2016; 138: 2570–2578.
 Visconti VV, Cariati I, Fittipaldi S, Iundusi R, Gasbarra E, Tarantino U, et al. DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review. International Journal of Molecular Sciences. 2021; 22: 4244.
 Hua-li Xu, Shi-yuan Xu. Tacrine alleviates neuropathic pain in mice by mimicking the cell adhesion molecule L1. Signa Vitae. 2021; 17: 140–150.
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