TfR associated with cervical cancer with or without neoadjuvant chemotherapy
1Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, 610041 Chengdu, Sichuan, China
2Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Sichuan University, 610041 Chengdu, Sichuan, China
3Department of Reproductive Medical Center, West China Second University Hospital, 610041 Chengdu, Sichuan, China
4West China Medical School, Sichuan University, 610041 Chengdu, Sichuan, China
5West China Laboratory of Molecular Genetics, Sichuan University, 610041 Chengdu, Sichuan, China
DOI: 10.22514/ejgo.2023.010 Vol.44,Issue 1,February 2023 pp.87-92
Submitted: 18 July 2022 Accepted: 19 October 2022
Published: 15 February 2023
*Corresponding Author(s): Tian Tang E-mail: firstname.lastname@example.org
† These authors contributed equally.
The objectives of this study was to examine the role of transferrin receptor (TfR) in the treatment of cervical cancer. Demographic and cancer-specific data were collected prospectively. Cancerous and adjacent mucosa tissues with neoadjuvant chemotherapy (NACT) or surgery alone were collected. TfR mRNA and protein expression was measured by Quantitative reverse transcription PCR (RT-qPCR) and immunoblots. We measured the cell viability by the MTT (3- [4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay with or without TfR-siRNA transfection. All 42 patients aged 49.31 ± 6.30 years with locally advanced cervical cancer were included: NACT (n = 22), surgery alone (n = 20). Quantitative results showed that the levels of TfR mRNA & protein in cancerous tissues were higher than those in adjacent mucosal tissues. We also demonstrated that the levels of TfR mRNA and protein were lower in tumor tissues collected from patients with NACT than in those collected from patients without NACT. We also observed that silencing of the TfR gene suppressed the survival of HeLa cells (48 h, p < 0.05). Our findings suggest a potential value for TfR as a diagnosis or evaluation marker for cervical cancer. The present study suggests that si-TfR may have an antitumou effect against cervical cancer cells, probably by limiting cell proliferation and reducing the TfR mRNA and protein levels.
Neoadjuvant chemotherapy; Transferrin receptor; Cervical cancer; Clinical characteristics
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