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Original Research

Open Access

Taraxasterol stimulates apoptosis of BCa cells via restraining PI3K/AKT and epidermal growth factor receptor (EGFR)

  • Yanjun Hu1
  • Xiping Zhang1,*,
  • Shuai Zhao1

1Department of breast surgery, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, 310022 Hangzhou, Zhejiang, China

DOI: 10.22514/ejgo.2024.007 Vol.45,Issue 1,February 2024 pp.42-49

Submitted: 31 August 2023 Accepted: 26 October 2023

Published: 15 February 2024

*Corresponding Author(s): Xiping Zhang E-mail:


Breast cancer (BCa) is a prevalent form of tumor, necessitating the development of more effective therapeutic drugs to improve patient prognosis. Taraxasterol (TAX), derived from the dandelion plant, has demonstrated significant physiological and pharmacological activity, but its role in BCa and underlying mechanism remains unexplored. In this study, we investigate the effects of TAX on the growth, motility and apoptosis of BCa cells in vitro to determine its mechanistic actions. Our results demonstrate that TAX significantly reduces both the proliferation and motility of BCa cells and induces apoptosis in BCa cells, as confirmed through flow cytometry (FCM). This apoptotic effect was further supported by JC-1 staining, which showed alterations in mitochondrial membrane potential in BCa cells after TAX treatment. Mechanistically, TAX suppresses the phosphatidylinositide 3-kinases/protein kinase B (PI3K/AKT) and EGFR pathways and impedes BCa progression. Collectively, TAX stimulates BCa cell apoptosis by inhibiting the PI3K/AKT and EGFR pathways.


BCa; Taraxasterol (TAX); Apoptosis; Mitochondrial membrane potential; PI3K/AKT

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Yanjun Hu,Xiping Zhang,Shuai Zhao. Taraxasterol stimulates apoptosis of BCa cells via restraining PI3K/AKT and epidermal growth factor receptor (EGFR). European Journal of Gynaecological Oncology. 2024. 45(1);42-49.


[1] Wang Y, Zhang C, Mai L, Niu Y, Wang Y, Bu Y. PRR11 and SKA2 gene pair is overexpressed and regulated by p53 in breast cancer. BMB Reports. 2019; 52: 157–162.

[2] Patra A, Satpathy S, Hussain MD. Nanodelivery and anticancer effect of a limonoid, nimbolide, in breast and pancreatic cancer cells. International Journal of Nanomedicine. 2019; 14: 8095–8104.

[3] Krämer S, Rogmans C, Saylan D, Friedrich D, Kraft C, Rogmans G, et al. Treatment progress in triple negative breast cancer. European Journal of Gynaecological Oncology. 2022; 43: 341–352.

[4] Bordignon MB, Pesce Viglietti AI, Julia EP, Sanchez MB, Rolle A, Mando P, et al. Phenotypic and functional analysis in HER2+ targeted therapy of human NK cell subpopulation according to the expression of FcεRIγ and NKG2C in breast cancer patients. Cancer Immunology, Immunotherapy. 2023; 72: 2687–2700.

[5] Lin KJ, Lengacher CA, Rodriguez CS, Szalacha LA, Wolgemuth J. Educational programs for post-treatment breast cancer survivors: a systematic review. European Journal of Gynaecological Oncology. 2022; 43: 285–314.

[6] Tang CT, Yang J, Liu ZD, Chen Y, Zeng C. Taraxasterol acetate targets RNF31 to inhibit RNF31/p53 axis-driven cell proliferation in colorectal cancer. Cell Death Discovery. 2021; 7: 66.

[7] Sang R, Ge B, Li H, Zhou H, Yan K, Wang W, et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. Ecotoxicology and Environmental Safety. 2023; 251: 114546.

[8] Ren F, Zhang Y, Qin Y, Shang J, Wang Y, Wei P, et al. Taraxasterol prompted the anti-tumor effect in mice burden hepatocellular carcinoma by regulating T lymphocytes. Cell Death Discovery. 2022; 8: 264.

[9] Lu J, Shuai B, Shou Z, Guo W, Zhou C, Ouyang X, et al. Taraxasterol inhibits tumor growth by inducing apoptosis and modulating the tumor microenvironment in non-small cell lung cancer. Cancers. 2022; 14: 4645.

[10] Ge B, Sang R, Wang W, Yan K, Yu Y, Kong L, et al. Protection of taraxasterol against acetaminophen-induced liver injury elucidated through network pharmacology and in vitro and in vivo experiments. Phytomedicine. 2023; 116: 154872.

[11] Li G, Zhang D, Wang S, Jie N, Qin Y. Protective role of Taraxasterol against cardiovascular aging and aging-induced desensitization of insulin signaling. Frontiers in Bioscience. 2022; 27: 311.

[12] Li C, Wang M, Chen X, Chen W. Taraxasterol ameliorates dextran sodium sulfate-induced murine colitis via improving intestinal barrier and modulating gut microbiota dysbiosis. Acta Biochimica Et Biophysica Sinica. 2022; 54: 340–349.

[13] Yin J, Lin Y, Fang W, Zhang X, Wei J, Hu G, et al. Tetrandrine citrate suppresses breast cancer via depletion of glutathione peroxidase 4 and activation of nuclear receptor coactivator 4-mediated ferritinophagy. Frontiers in Pharmacology. 2022; 13: 820593.

[14] Karginova O, Weekley CM, Raoul A, Alsayed A, Wu T, Lee SS, et al. Inhibition of copper transport induces apoptosis in triple-negative breast cancer cells and suppresses tumor angiogenesis. Molecular Cancer Therapeutics. 2019; 18: 873–885.

[15] Pan Y, Zhang Q, Zhang H, Kong F. Prognostic and immune microenvironment analysis of cuproptosis-related LncRNAs in breast cancer. Functional & Integrative Genomics. 2023; 23: 38.

[16] Luan Y, Ren KD, Luan Y, Chen X, Yang Y. Mitochondrial dynamics: pathogenesis and therapeutic targets of vascular diseases. Frontiers in Cardiovascular Medicine. 2021; 8: 770574.

[17] Luan Y, Luan Y, Yuan R, Feng Q, Chen X, Yang Y. Structure and function of mitochondria-associated endoplasmic reticulum membranes (MAMs) and their role in cardiovascular diseases. Oxidative Medicine and Cellular Longevity. 2021; 2021: 1–19.

[18] Ye X, Xu R, Liu S, Hu B, Shi Z, Shi F, et al. Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes. International Immunopharmacology. 2022; 102: 108380.

[19] Wang J, Zheng K, Jin Y, Fu Y, Wang R, Zhang J. Protective effects of taraxasterol against deoxynivalenol-induced damage to bovine mammary epithelial cells. Toxins. 2022; 14: 211.

[20] Liu W, Yu Q, Wang F, Li Y, Zhang G, Tao S. Taraxasterol attenuates melanoma progression via inactivation of reactive oxygen species-mediated PI3K/Akt signaling pathway. Human & Experimental Toxicology. 2022; 41: 09603271211069034.

[21] Li H, Sang R, Zhao X, Li C, Wang W, Wang M, et al. Research note: Taraxasterol alleviates aflatoxin B1-induced oxidative stress in chicken primary hepatocytes. Poultry Science. 2023; 102: 102286.

[22] Yang J, Xin C, Yin G, Li J. Taraxasterol suppresses the proliferation and tumor growth of androgen-independent prostate cancer cells through the FGFR2-PI3K/AKT signaling pathway. Scientific Reports. 2023; 13: 13072.

[23] Xie Z, Wang B, Zheng C, Qu Y, Xu J, Wang B, et al. Taraxasterol inhibits inflammation in osteoarthritis rat model by regulating miRNAs and NF-kB signaling pathway. Acta Biochimica Polonica. 2022; 69: 811–818.

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