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Original Research

Open Access

The mechanism of miR-143-3p regulating FGF-9 in the proliferation, invasion

  • Wang Yan1,*,

1Tangshan People’s Hospital, 063000 Tangshan, Hebei, China

DOI: 10.22514/ejgo.2024.009 Vol.45,Issue 1,February 2024 pp.55-62

Submitted: 29 August 2022 Accepted: 29 November 2022

Published: 15 February 2024

*Corresponding Author(s): Wang Yan E-mail: hblgdxzmhxf@nest.edu.cn

Abstract

The study aimed at exploring the miR-143-3p expression and the mechanism involved in ovarian cancer development. RT-qPCR (Quantitative Real-time PCR) served for the detection of the miR-143-3p level in different ovarian carcinoma cells. Then miR-143-3p underwent transfection and presented overexpression in ovarian carcinoma cells. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, flat cloning experiment, Transwell invasion assay and scratch test served for the evaluation of the influences of miR-143-3p on ovarian cancer cell biological behavior. The candidate target genes were screened by using bioinformatics method. The double luciferase assay and Western blot together assessed the regulatory effect of miR-143-3p. Relative to the control group (NC), the overexpressed group presented significantly reduced ovarian cancer cell growth (p < 0.01). The results indicated the inhibitory impact of miR-143-3p overexpression on the invasion and migration of ovarian cancer 3AO and SKOV3 cells. Bioinformatics analysis confirmed FGF-9 (Fibroblast Growth Factor 9) as one of target gene of miR-143-3p. Double luciferase assay also attested the direct negative regulatory impact of miR-143-3p on FGF-9 (p < 0.05). The miR-143-3p overexpression significantly lowered the protein level of FGF-9 in 3AO and SKOV3 cells (p < 0.001). miR-143-3p overexpression negatively regulated the proliferation, invasion and migration abilities of ovarian carcinoma cells, as well as the FGF-9 protein expression.


Keywords

miR-143-3p; Ovarian carcinoma; Proliferation; Invasion; Migration; FGF-9


Cite and Share

Wang Yan. The mechanism of miR-143-3p regulating FGF-9 in the proliferation, invasion. European Journal of Gynaecological Oncology. 2024. 45(1);55-62.

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