Article Data

  • Views 917
  • Dowloads 123

Original Research

Open Access

Magnoflorine inhibits cisplatin-induced protective autophagy by down-regulating HMGB1 and increases drug sensitivity in drug-resistant ovarian cancer cells

  • Yiling Zhang1,†
  • Xin He2,†
  • Lei Fan3,*,
  • Qianru Zhang4,*,

1Department of Oncology, Xi’an Daxing Hospital, 710016 Xi’an, Shaanxi, China

2Department of Oncology, Tangdu Hospital, Air Force Medical University, 710038 Xi’an, Shaanxi, China

3Department of Pharmacy, The Second Affiliated Hospital of Zhejiang Chinese Medical University, 310005 Hangzhou, Zhejiang, China

4Department of Pharmacy, Xi’an Daxing Hospital, 710016 Xi’an, Shaanxi, China

DOI: 10.22514/ejgo.2024.018 Vol.45,Issue 1,February 2024 pp.132-138

Submitted: 07 October 2023 Accepted: 29 November 2023

Published: 15 February 2024

*Corresponding Author(s): Lei Fan E-mail: fanlei3092701@163.com
*Corresponding Author(s): Qianru Zhang E-mail: audrey629@126.com

† These authors contributed equally.

Abstract

Ovarian cancer (OC) is a prevalent type of gynecologic malignancy, and to improve its treatment effectiveness, it is essential to identify new and more potent therapeutic drugs. Magnoflorine is a fundamental chlorine alkaloid with various pharmacological properties, including anti-diabetic and anti-inflammatory effects. However, whether Magnoflorine can inhibit the protective autophagy induced by cisplatin by modulating (high-mobility group B1) HMGB1 requires further investigation. This study aims to assess the impact of Magnoflorine on cisplatin resistance in OC. Our findings demonstrate that Magnoflorine enhances the sensitivity of OC cells to cisplatin. Additionally, it promotes apoptosis in cisplatin-resistant OC cells and simultaneously inhibits cisplatin-induced protective autophagy. Mechanistically, Magnoflorine reduces HMGB1 expression in cisplatin-resistant OC cells. Collectively, these results suggest that Magnoflorine has potential as a therapeutic agent for cisplatin-resistant OC in future clinical applications.


Keywords

Ovarian cancer; Cisplatin; Apoptosis; Autophagy; HMGB1


Cite and Share

Yiling Zhang,Xin He,Lei Fan,Qianru Zhang. Magnoflorine inhibits cisplatin-induced protective autophagy by down-regulating HMGB1 and increases drug sensitivity in drug-resistant ovarian cancer cells. European Journal of Gynaecological Oncology. 2024. 45(1);132-138.

References

[1] Gloss B, Moran-Jones K, Lin V, Gonzalez M, Scurry J, Hacker NF, et al. ZNF300P1 Encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer. Molecular Cancer. 2014; 13: 3.

[2] Pickwell-Smith B, Greenley S, Lind M, Macleod U. Where are the inequalities in ovarian cancer care in a country with universal healthcare? A systematic review and narrative synthesis. Journal of Cancer Policy. 2023; 39: 100458.

[3] Ma Y, Wei Z, Bast RC, Wang Z, Li Y, Gao M, et al. Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo. Laboratory Investigation. 2016; 96: 37–48.

[4] He H, Feng Y, Zang L, Liu W, Ding L, Chen L, et al. Nitric oxide induces apoptosis and autophagy; autophagy down-regulates no synthesis in physalin A-treated a375-S2 human melanoma cells. Food and Chemical Toxicology. 2014; 71: 128–135.

[5] He J, Zhang G, Pang Q, Yu C, Xiong J, Zhu J, et al. SIRT6 reduces macrophage foam cell formation by inducing autophagy and cholesterol efflux under ox-LDL condition. The FEBS Journal. 2017; 284: 1324–1337.

[6] Tan J, Jiang X, Yin G, He L, Liu J, Long Z, et al. Anacardic acid induces cell apoptosis of prostatic cancer through autophagy by ER stress/DAPK3/Akt signaling pathway. Oncology Reports. 2017; 38: 1373–1382.

[7] Ghezelayagh TS, Wu ES, Barber EL, Dao MD, Zsiros E, Urban RR, et al. Timing and duration of bevacizumab treatment and survival in patients with recurrent ovarian, fallopian tube, and peritoneal cancer: a multi-institution study. European Journal of Gynaecological Oncology. 2023; 44: 17–25.

[8] Chen CD, Dai LN, Wang M, Lai XH, Wang J. Hypoxia induces autophagy in PA-l ovarian teratoma cells and resistance to growth inhibition and apoptosis by chemotherapeutic agent cis-diamminedichloroplatinum. European Journal of Gynaecological Oncology. 2017; 38: 277–281.

[9] Zhu J, Lin S, Zou X, Chen X, Liu Y, Yang X, et al. Mechanisms of autophagy and endoplasmic reticulum stress in the reversal of platinum resistance of epithelial ovarian cancer cells by naringin. Molecular Biology Reports. 2023; 50: 6457–6468.

[10] Okon E, Kukula-Koch W, Halasa M, Jarzab A, Baran M, Dmoszynska-Graniczka M, et al. Magnoflorine-isolation and the anticancer potential against NCI-H1299 Lung, MDA-MB-468 Breast, T98G Glioma, and TE671 rhabdomyosarcoma cancer cells. Biomolecules. 2020; 10: 1532.

[11] Yadav A, Singh A, Phogat J, Dahuja A, Dabur R. Magnoflorine prevent the skeletal muscle atrophy via Akt/mTOR/FoxO signal pathway and increase slow-MyHC production in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology. 2021; 267: 113510.

[12] Sun X, Zhang X, Zhai H, Zhang D, Ma S. Magnoflorine inhibits human gastric cancer progression by inducing autophagy, apoptosis and cell cycle arrest by JNK activation regulated by ROS. Biomedicine & Pharmacotherapy. 2020; 125: 109118.

[13] Wei T, Xiaojun X, Peilong C. Magnoflorine improves sensitivity to doxorubicin (DOX) of breast cancer cells via inducing apoptosis and autophagy through AKT/mTOR and p38 signaling pathways. Biomedicine & Pharmacotherapy. 2020; 121: 109139.

[14] Xu T, Jiang L, Wang Z. The progression of HMGB1-induced autophagy in cancer biology. OncoTargets and Therapy. 2018; 12: 365–377.

[15] Burges A, Schmalfeldt B. Ovarian cancer: diagnosis and treatment. Deutsches Ärzteblatt International. 2011; 108: 635–641.

[16] Zhong L, Qin Y, Liu M, Sun J, Tang H, Zeng Y, et al. Magnoflorine improves cognitive deficits and pathology of Alzheimer’s disease via inhibiting of JNK signaling pathway. Phytomedicine. 2023; 112: 154714.

[17] Wang L, Li P, Zhou Y, Gu R, Lu G, Zhang C. Magnoflorine ameliorates collagen-induced arthritis by suppressing the inflammation response via the NF-kB/MAPK signaling pathways. Journal of Inflammation Research. 2023; 16: 2271–2296.

[18] Liang H, Chang X, Xia R, Wu W, Guo H, Yang M. Magnoflorine attenuates cerebral ischemia-induced neuronal injury via autophagy/sirt1/AMPK signaling pathway. Evidence-Based Complementary and Alternative Medicine. 2022; 2022: 1–11.

[19] Zhang L, Liu Y, Lei X, Liu X, Sun H, Liu S. Astragaloside II enhanced sensitivity of ovarian cancer cells to cisplatin via triggering apoptosis and autophagy. Cell Biology International. 2023; 47: 1600–1613.

[20] Zhou Y, Liu T, Wu Q, Wang H, Sun Y. Baohuoside I inhibits resistance to cisplatin in ovarian cancer cells by suppressing autophagy via downregulating HIF-1a/ATG5 axis. Molecular Carcinogenesis. 2023; 62: 1474–1486.

[21] Baran M, Miziak P, Bonio K. Magnoflorsine—a compound with anti-tumour activity. Journal of Pre-Clinical and Clinical Research. 2020; 14: 98–101.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,500 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Biological Abstracts Easily discover critical journal coverage of the life sciences with Biological Abstracts, produced by the Web of Science Group, with topics ranging from botany to microbiology to pharmacology. Including BIOSIS indexing and MeSH terms, specialized indexing in Biological Abstracts helps you to discover more accurate, context-sensitive results.

Google Scholar Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines.

JournalSeek Genamics JournalSeek is the largest completely categorized database of freely available journal information available on the internet. The database presently contains 39226 titles. Journal information includes the description (aims and scope), journal abbreviation, journal homepage link, subject category and ISSN.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

BIOSIS Previews BIOSIS Previews is an English-language, bibliographic database service, with abstracts and citation indexing. It is part of Clarivate Analytics Web of Science suite. BIOSIS Previews indexes data from 1926 to the present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Submission Turnaround Time

Conferences

Top