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Original Research

Open Access

Sufentanil inhibits the invasion and epithelial-mesenchymal transition of endometrial cancer cells in vitro

  • Hongxia Chen1,†
  • Guangquan He2,†
  • Qin Gao1,*,

1Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, 637000 Nanchong, Sichuan, China

2Department of Anesthesiology, Nanchong Central Hospital, 637000 Nanchong, Sichuan, China

DOI: 10.22514/ejgo.2024.033 Vol.45,Issue 2,April 2024 pp.112-117

Submitted: 01 November 2023 Accepted: 13 December 2023

Published: 15 April 2024

*Corresponding Author(s): Qin Gao E-mail:

† These authors contributed equally.


This study investigates the impact of sufentanil on endometrial cancer (EC) cells. Human EC cell lines (HEC-1A and Ishikawa) were exposed to different concentrations (10, 20 and 40 nM) of sufentanil for 48 hours, following which cell-counting-kit 8 (CCK8), clone formation and transwell assays as well as and western blot were conducted. The findings revealed that sufentanil reduced the cell viability, colony formation, migration and invasion of both HEC-1A and Ishikawa cells. Moreover, sufentanil treatment resulted in decreased levels of matrix metallo proteinase 9 (MMP-9), MMP-2, N-cadherin and alpha smooth muscle actin (α-SMA) proteins while increasing the expression of E-cadherin in treated cells. Furthermore, sufentanil treatment was associated with decreased phosphorylation of phosphatidylinositol 3-kinase (PI3K), Akt and mammalian target of rapamycin (mTOR), suggesting that its inhibitory effects on the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of EC cells could be attributed to the inactivation of the PI3K/Akt/mTOR pathway.


Endometrial cancer; Sufentanil; PI3K/Akt/mTOR pathway; Migration; Invasion; EMT

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Hongxia Chen,Guangquan He,Qin Gao. Sufentanil inhibits the invasion and epithelial-mesenchymal transition of endometrial cancer cells in vitro. European Journal of Gynaecological Oncology. 2024. 45(2);112-117.


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