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Original Research

Open Access

In vitro effects of osteopontin on endometrial cancer cells and signaling pathways in epithelial-mesenchymal transition

  • Evrim Kardelen1,*,
  • Tolga Atakul2,*,
  • Hasan Yüksel3,*,

1Department of Obstetrics and Gynecology, Çiğli Training and Research Hospital, 35620 İzmir, Turkey

2Department of Obstetrics and Gynecology, Private Obstetrics and Gynecology Clinic, 09100 Aydın, Turkey

3Department of Obstetrics and Gynecology, Division of Gynecological Oncology, Aydın Adnan Menderes University Faculty of Medicine, 09010 Aydın, Turkey

DOI: 10.22514/ejgo.2024.103 Vol.45,Issue 5,October 2024 pp.152-161

Submitted: 12 September 2023 Accepted: 13 December 2023

Published: 15 October 2024

*Corresponding Author(s): Evrim Kardelen E-mail: ekardelen@adu.edu.tr
*Corresponding Author(s): Tolga Atakul E-mail: tolgaatakul@uskudar.edu.tr
*Corresponding Author(s): Hasan Yüksel E-mail: hyuksel@adu.edu.tr

Abstract

This study aims to demonstrate in vitro effects of osteopontin (OPN), an extracellular matrix molecule, on proliferation and migration in human umbilical vein endothelial cells (HUVEC) and Ishikawa human endometrial adenocarcinoma cells (Ishikawa) cultures. In addition, the effects of OPN on phosphatidylinositol 3 kinase (PI3K) and extracellular signal regulatory kinase 1/2 (ERK1/2), which are involved signaling pathways in cell cycle, were investigated. This research was carried out as a prospective cell culture study in Aydın Adnan Menderes University Biochemistry Laboratory. Two types of cell culture were used, HUVEC representing endothelial cells and Ishikawa representing tumour cells. The HUVEC and Ishikawa were extracted from liquid nitrogen and seeded in culture medium. With the increasing concentrations of recombinant human OPN (rhOPN) in both cell lines, proliferation and migration of cells and PI3K and ERK1/2 levels in the medium were investigated. Detection of cell proliferation in these two cell types with MTT test, and migration assay were performed. Proliferation was found to increase in both cell types with increasing levels of rhOPN (p < 0.01). In both cell cultures, rhOPN induced mesenchymal transformation, indicating migration at the highest (400 ng/mL) concentrations. Increasing levels of PI3K and ERK1/2 were measured with the increasing concentrations of rhOPN (p < 0.01 and p < 0.05, respectively). Proliferation, migration, and PI3K and ERK1/2 molecules are increased with rhOPN in both cell cultures studied. This suggests that OPN could be a candidate prognostic marker in endometrial cancer.


Keywords

Endometrial cancer; Extracellular signal regulatory kinase 1/2; Human umbilical vein endothelial cells; Ishikawa cells; Osteopontin; Phosphatidylinositol 3 kinase


Cite and Share

Evrim Kardelen,Tolga Atakul,Hasan Yüksel. In vitro effects of osteopontin on endometrial cancer cells and signaling pathways in epithelial-mesenchymal transition. European Journal of Gynaecological Oncology. 2024. 45(5);152-161.

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