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Downregulation of α-enolase (ENO1) Inhibits Growth, Invasion, and Metastasis of Human Cervical Cancer Cells
1Department of Maternal and Child Health Hospital of Zibo City, Zibo, Shandong, 255029, P. R. China.
2Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
3Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China
4ICU, Huantai County People’s Hospital, Zibo, Shandong, 256400, P. R. China.
5Department of Medical Oncology, Gansu Provincial Tumor Hospital, Lanzhou, Gansu, 730000, P. R. China
6Institute of Human Anatomy, Histology and Embryology, school of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China
7Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, United States 8Department of Obstetrics and Gynecology, Gansu Provincial People’s Hospital, Lanzhou, Gansu, 730000, P. R. China
DOI: 10.31083/j.ejgo.2020.05.5314 Vol.41,Issue 5,October 2020 pp.762-768
Submitted: 18 June 2019 Accepted: 15 June 2020
Published: 15 October 2020
*Corresponding Author(s): Yuzhen Guo E-mail: guoyz@lzu.edu.cn
Objectives: The aerobic glycolysis is a characteristic of cancer. α-enolase (ENO1) is an important glycolytic enzyme and is suggested to play a role in the development of tumor. The purpose of this study was to investigate the effects of ENO1 on growth, invasion, and metastasis of human cervical cancer cells. Methods: We knocked down gene coding ENO1 in Hela and SiHa cells with lentivirus, which was confirmed by western-blot. Following downregulation of ENO1, the drug sensitivity, invasion, metastasis, tumourigenic abilities of ENO1-altered cells were investigated. Results: Our studies demonstrated that downregulation of ENO1 enhanced the sensitivity of Hela and SiHa cells to paclitaxel and cisplatin and decreased their tumorigenicity. In addition, the abilities of invasion, metastasis, and tumourigenesis were decreased in ENO1-silenced Hela and SiHa cells. Conclusions: ENO1 is involved in the tumorigenicity, invasion, metastasis, and drug sensitivity of Hela and SiHa cells.
α-enolase; Drug sensitivity; Cell tumorigenicity; Invasion and metastasis.
Dongdong Zhang,Bingdong Zhu,Yajie Fu,Yin Han,Yufeng Wang,Xiaoyun Chen,Lang Zhang,Xiangwen Liu,Ying Zhang,Yuzhen Guo,Huiling Liu. Downregulation of α-enolase (ENO1) Inhibits Growth, Invasion, and Metastasis of Human Cervical Cancer Cells. European Journal of Gynaecological Oncology. 2020. 41(5);762-768.
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