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The importance of dysregulated miRNAs on ovarian cysts and epithelial ovarian cancer

  • Ece Gumusoglu1
  • Tuba Gunel1,*,
  • Mohammad Kazem Hosseini1
  • Nogayhan Seymen2
  • Taylan Senol3
  • Uğur Sezerman2
  • Samet Topuz4
  • Kılıç Aydınlı5

1Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Istanbul, Turkey

2Acıbadem University, School of Medicine, Department of Basic Sciences, Biostatistics, 34684, Istanbul Turkey

3Istanbul Zeynep Kamil Women and Child Diseases Training and Research Hospital, 34668, Istanbul, Turkey

4Istanbul University, Istanbul Medical Faculty, Department of Obstetrics and Gynecology, 34093, Istanbul, Turkey

5Medicus Healthcare Centre, 34365, Istanbul, Turkey

DOI: 10.31083/j.ejgo.2021.01.2167 Vol.42,Issue 1,February 2021 pp.66-72

Submitted: 09 June 2020 Accepted: 16 October 2020

Published: 15 February 2021

*Corresponding Author(s): Tuba Gunel E-mail:


Objective: Benign ovarian cysts (BOC) are the most common tumors in women of reproductive age. Usually, these cysts are harmless, but, a small number of them occasionally progress to malignancy. Among ovarian malignancies, epithelial ovarian cancer (EOC) comprises 90% and is the most important cause of gynecologic cancer-related deaths. We aimed to identify dysregulated miRNAs in patients with benign ovarian cysts (n = 11) compared to EOC (n = 10) and to healthy individuals (HI) (n = 15). Methods: The serum samples from EOC and BOC patients were collected before operation. We studied three different sample groups (serum of EOC (n = 8), HI (n = 8), and BOC (n = 8) patients) that contained the highest-quality of RNA. Microarray data were analyzed according to expression of miRNAs and target genes by bioinformatics tools. Results: When compared to EOC samples, 75 miRNAs were dysregulated in BOC samples. Sixty-six miRNAs from BOC were dysregulated when compared to HI samples. Bioinformatics analysis of BOC vs. EOC and BOC vs. HI showed that 46 miRNAs were congruent and their expression alterations were similar (up- or down-regulated). Further analysis showed that these 46 miRNAs are associated to one of three pathways involved in cancer pathogenesis. Conclusion: Several miRNAs might play a role in BOC formation and/or malignant transformation. These dysregulated miRNAs could potentially be a biomarker to distinguish between a completely BOC and one that is malignant or has potential for malignant transformation.


Ovarian neoplasms; Ovarian cysts; MicroRNAs; Neoplastic transformation; Ep-ithelial ovarian cancer

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Ece Gumusoglu,Tuba Gunel,Mohammad Kazem Hosseini,Nogayhan Seymen,Taylan Senol,Uğur Sezerman,Samet Topuz,Kılıç Aydınlı. The importance of dysregulated miRNAs on ovarian cysts and epithelial ovarian cancer. European Journal of Gynaecological Oncology. 2021. 42(1);66-72.


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