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Prognostic value of matrix metalloproteinase 2 protein expression in ovarian cancer is age- and stage-dependent

  • Mourad Assidi1,2
  • Mohammad Alam Jafri1,2
  • Muhammad Abu-Elmagd1,2
  • Salina Saddi3
  • Safia Messaoudi4
  • Mahmood Rasool1,2
  • Jaudah AlMaghrabi5
  • Nisreen Anfinan6
  • Hanen Chelbi7
  • Maram Sait8
  • Abdelfatteh El Omri9
  • Hesham Sait6
  • Hussain Basalamah6
  • Khalid Sait6
  • Abdelbaset Buhmeida1,*,

1Center of Excellence in Genomic Medicine Research, King Abdulaziz University, 21589 Jeddah, Saudi Arabia

2Medical Laboratory Department, Faculty of Applied Medical Sciences, King Abdulaziz University, 21589 Jeddah, Saudi Arabia

3Biological Sciences Department, Faculty of Science, King Abdulaziz University, 21589 Jeddah, Saudi Arabia

4Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, 14812 Riyadh, Saudi Arabia

5Department of Pathology, Faculty of Medicine, King Abdulaziz University Hospital, 21589 Jeddah, Saudi Arabia

6Department of Obstetrics and Gynecology, Faculty of Medicine, King Abdulaziz University Hospital, 21589 Jeddah, Saudi Arabia

7Laboratoire de Parasitologie-Mycologie, Pasteur Institute of Tunis, 1002 Tunis, Tunisia

8Faculty of Medicine, King Abdulaziz University Hospital, 21589 Jeddah, Saudi Arabia

9Surgical Research Section, Department of Surgery, Hamad Medical Corporation, 55867 Doha, Qatar

DOI: 10.31083/j.ejgo4301010 Vol.43,Issue 1,February 2022 pp.26-35

Submitted: 12 September 2021 Accepted: 17 November 2021

Published: 15 February 2022

*Corresponding Author(s): Abdelbaset Buhmeida E-mail:


Objective: Matrix metalloproteinase 2 (MMP2) has been associated with tumor development and invasion; however, the information available regarding its prognostic value in ovarian cancer (OC), especially in the Arabian Peninsula, is limited. The aim of this retrospective study was to analyze MMP2 protein expression and assess its prognostic value. Methods: In total, 245 formalin-fixed and paraffin-embedded (FFPE) primary OC tissue samples were randomly collected from patients with available clinicopathological data, including disease of all stages and all histological subtypes. MMP2 protein expression was measured using automated tissue microarray and immunohistochemistry techniques. Statistical analyses were performed using SPSS, with p << 0.05 considered statistically significant. Results: Cytoplasmic MMP2 protein expression patterns were higher in 53% of all tumor samples. The MMP2 expression profile was not significantly correlated with most clinicopathological features including age, tumor site, size, grade, and lymph node status (p >> 0.05). However, when adjusted according to the disease stage or patient age, MMP2 overexpression showed a significant indication of a poor outcome and recurrence as evaluated using univariate Kaplan–Meier analysis for disease-free survival (DFS) (p = 0.04 and p = 0.03, respectively, log-rank test), but not for disease-specific survival (DSS) (p >> 0.05, log-rank test). Conclusion: This study showed that MMP2 protein overexpression was a negative prognosticator in Saudi OC patients with advanced stage and/or young age. These results could pave the way towards more effective and personalized detection, prognosis, and management of OC.


Ovarian cancer; Matrix metalloproteinase; MMP2; Prognosis; Tissue microarray; Age; Stage

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Mourad Assidi,Mohammad Alam Jafri,Muhammad Abu-Elmagd,Salina Saddi,Safia Messaoudi,Mahmood Rasool,Jaudah AlMaghrabi,Nisreen Anfinan,Hanen Chelbi,Maram Sait,Abdelfatteh El Omri,Hesham Sait,Hussain Basalamah,Khalid Sait,Abdelbaset Buhmeida. Prognostic value of matrix metalloproteinase 2 protein expression in ovarian cancer is age- and stage-dependent. European Journal of Gynaecological Oncology. 2022. 43(1);26-35.


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