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Original Research

Open Access

Huwe1 inactivation promotes ovarian cancer metastasis by extracellular matrix deregulation

  • Fujuan Zhang1,†
  • Jing Guo1,†
  • Shuaishuai Yu1
  • Xudong Zhao1,2
  • Yongxin Ma1,*,
  • Dong Yang1,2,*,

1Laboratory of Animal Tumor Models, Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy andCancer Center, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, China

2Kunming Institute of Zoology, Chinese Academy of Sciences, 650223 Kunming, Yunnan, China

DOI: 10.31083/j.ejgo4301013 Vol.43,Issue 1,February 2022 pp.48-55

Submitted: 12 October 2021 Accepted: 29 November 2021

Published: 15 February 2022

*Corresponding Author(s): Yongxin Ma E-mail:
*Corresponding Author(s): Dong Yang E-mail:

† These authors contributed equally.


Objective: Huwe1 is critical for promoting the progression of a range of malignancies including ovarian cancer. Still, its role in ovarian cancer metastasis has not been reported. The goal of our study was to evaluate a correlation between Huwe1 and ovarian cancer metastasis. Methods: The mouse ovary surface epithelium (MOSE) cells isolated from Huwe1L/LL/L mice were identified by flow cytometry assay. Cell migration and invasion were analyzed by transwell system. The molecular mechanism of Huwe1 in ovarian cancer migration and invasion was explored by analyzing the difference of RNAseq data between MOSE-Huwe1L/LL/L-CreER and MOSE-Huwe1L/LL/L-Cre cells. Results: Huwe1 deletion significantly promoted tumor migration and invasion in ovarian cancer cells. Moreover, tumor cells were more frequently detected in the blood when the mice bearing allografts were treated with tamoxifen. Transcriptome analysis indicated that this phenotype may be due to the alteration in cell adhesion caused by the Huwe1 knockout. Conclusion: Huwe1 inactivation promoted ovarian cancer metastasis by the extracellular matrix (ECM) deregulation.


Huwe1; Invasion; Ovarian cancer; MOSE cells; Extracellular matrix

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Fujuan Zhang,Jing Guo,Shuaishuai Yu,Xudong Zhao,Yongxin Ma,Dong Yang. Huwe1 inactivation promotes ovarian cancer metastasis by extracellular matrix deregulation. European Journal of Gynaecological Oncology. 2022. 43(1);48-55.


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